Analytical Data
-
Gene name
PGBD4
- Application
-
Alternative Names
FLJ32638; FLJ37497; PGBD4; PGBD4_HUMAN; PiggyBac transposable element-derived protein 4
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q96DM1
-
Expression Region
1-585 aa
-
AA Sequence
MSNPRKRSIP MRDSNTGLEQ LLAEDSFDES DFSEIDDSDN FSDSALEADK IRPLSHLESD GKSSTSSDSG RSMKWSARAM IPRQRYDFTG TPGRKVDVSD ITDPLQYFEL FFTEELVSKI TRETNAQAAL LASKPPGPKG FSRMDKWKDT DNDELKVFFA VMLLQGIVQK PELEMFWSTR PLLDTPYLRQ IMTGERFLLL FRCLHFVNNS SISAGQSKAQ ISLQKIKPVF DFLVNKFSTV YTPNRNIAVD ESLMLFKGPL AMKQYLPTKR VRFGLKLYVL CESQSGYVWN ALVHTGPGMN LKDSADGLKS SRIVLTLVND LLGQGYCVFL DNFNISPMLF RELHQNRTDA VGTARLNRKQ IPNDLKKRIA KGTTVARFCG ELMALKWCDG KEVTMLSTFH NDTVIEVNNR NGKKTKRPRV IVDYNENMGA VDSADQMLTS YPSERKRHKV WYKKFFHHLL HITVLNSYIL FKKDNPEHTM SHINFRLALI ERMLEKHHKP GQQHLRGRPC SDDVTPLRLS GRHFPKSIPA TSGKQNPTGR CKICCSQYDK DGKKIRKETR YFCAECDVPL CVVPCFEIYH TKKNY
-
Molecular Weight
67.0 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PGBD4, or PiggyBac Transposase 4, is a member of the PiggyBac transposon family, which has garnered significant attention in the field of molecular biology due to its unique ability to mediate genetic integration. This protein is mainly studied for its roles in gene therapy, transgenic animal production, and genome engineering due to its high efficiency in inserting DNA into host genomes. Research on PGBD4 has gained momentum as scientists seek to exploit its transpositional capabilities for therapeutic applications, including the correction of genetic defects and the development of new models for studying diseases. Recently, studies have indicated that PGBD4 is involved in cellular processes such as proliferation and differentiation, raising questions about its potential implications in cancer biology and regenerative medicine. The interest in PGBD4 is further driven by advances in synthetic biology, where understanding its structure and function could lead to tailored applications in gene delivery systems and precise genome editing techniques. Overall, ongoing research into PGBD4's mechanisms and applications aims to harness its potent transpositional activity to revolutionize genetic engineering and therapeutic strategies, making it a pivotal subject of investigation in contemporary biomedical research.
