Analytical Data
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Gene name
APOE
- Application
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Alternative Names
APOE；ApolipoProtein E
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P02649
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Expression Region
1-317aa
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AA Sequence
MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH
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Molecular Weight
34 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Apolipoprotein E (APOE) is a crucial protein involved in lipid metabolism and neuronal repair in the central nervous system. It exists in three major isoforms—APOE ε2, ε3, and ε4—which genetically influence individual susceptibility to Alzheimer's disease (AD). Research has shown that the APOE ε4 allele is a significant risk factor for AD, highlighting its role in disease pathophysiology. The varying effects of these isoforms on cholesterol transport, amyloid-beta aggregation, and neuroinflammation have piqued the interest of scientists, leading to extensive studies on APOE's structure and function. The characterization of recombinant forms of APOE has enabled further understanding of its biological roles and interactions, particularly in the context of neurodegeneration and cognitive decline. Investigating the biochemical properties and therapeutic potentials of these recombinant proteins could lead to new strategies for AD prevention and treatment. As such, APOE research bridges genetics, neurobiology, and therapeutic development, making it a vital area of inquiry in modern biomedical sciences.
