Analytical Data
-
Gene name
PIP5K1B
- Application
-
Alternative Names
MSS 4; MSS4; Phosphatidylinositol 4 phosphate 5 kinase type 1 beta; Phosphatidylinositol-4-phosphate 5-kinase type I beta; Phosphatidylinositol-4-phosphate 5-kinase type-1 beta; PI4P5K 1 beta; PI51B_HUMAN; PIP5K1-beta; PIP5K1B; PIP5KIbeta; Pipk 5 beta; Pipk 5b; Protein STM 7; Protein STM-7; PtdIns(4)P-5-kinase 1 beta; STM 7; STM7; STM7 LSB; Type I phosphatidylinositol 4 phosphate 5 kinase beta; Type I phosphatidylinositol-4-phosphate 5-kinase beta
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O14986
-
Expression Region
1-540 aa
-
AA Sequence
MSSAAENGEA APGKQNEEKT YKKTASSAIK GAIQLGIGYT VGNLTSKPER DVLMQDFYVV ESVFLPSEGS NLTPAHHYPD FRFKTYAPLA FRYFRELFGI KPDDYLYSIC SEPLIELSNP GASGSLFFVT SDDEFIIKTV QHKEAEFLQK LLPGYYMNLN QNPRTLLPKF YGLYCMQSGG INIRIVVMNN VLPRSMRMHF TYDLKGSTYK RRASRKEREK SNPTFKDLDF LQDMHEGLYF DTETYNALMK TLQRDCRVLE SFKIMDYSLL LGIHFLDHSL KEKEEETPQN VPDAKRTGMQ KVLYSTAMES IQGPGKSGDG IITENPDTMG GIPAKSHRGE KLLLFMGIID ILQSYRLMKK LEHSWKALVY DGDTVSVHRP SFYADRFLKF MNSRVFKKIQ ALKASPSKKR CNSIAALKAT SQEIVSSISQ EWKDEKRDLL TEGQSFSSLD EEALGSRHRP DLVPSTPSLF EAASLATTIS SSSLYVNEHY PHDRPTLYSN SKGLPSSSTF TLEEGTIYLT AEPNTLEVQD DNASVLDVYL
-
Molecular Weight
61.0 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIP5K1B, or Phosphatidylinositol-4,5-bisphosphate 1-kinase beta, is an important enzyme involved in lipid signaling pathways, particularly in the synthesis of phosphatidylinositol-4,5-bisphosphate (PIP2), a key regulator of various intracellular processes, including cell migration, proliferation, and apoptosis. Aberrant expression or dysfunction of PIP5K1B has been implicated in several diseases, including cancer and neurodegenerative disorders, making it a potential therapeutic target. Research into recombinant PIP5K1B protein aims to elucidate its structure-function relationship, understand the regulatory mechanisms governing its activity, and explore its role in cellular signaling networks. By generating a stable and functional recombinant form of PIP5K1B, scientists can perform detailed biochemical assays, structural characterization, and functional studies to investigate its interaction with other cellular proteins and lipids. These studies are crucial for developing new strategies for modulating PIP5K1B activity in pathological conditions and may contribute to advancing our understanding of phosphoinositide signaling pathways in health and disease. Additionally, recombinant PIP5K1B can serve as a valuable tool for drug screening and identifying small molecules that can selectively inhibit or activate this kinase, ultimately leading to novel therapeutic approaches for diseases associated with its dysregulation.
