Analytical Data
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Gene name
PNLDC1
- Application
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Alternative Names
oly(A)-specific ribonuclease PARN-like domain-containing protein 1; PNDC1_HUMAN; PNLDC1; poly(A)-specific ribonuclease (PARN)-like domain containing 1; Poly(A)-specific ribonuclease PARN-like domain-containing protein 1; RP1-195P10.1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8NA58
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AA Sequence
MFCTRGLLFFAFLAGLDIEFTGLRSNLSGPQQISLFDLPSEWYLKTRQSVQQFTVCQIGLSVFSAIEGEANKYIAHSCNFYLFPTTFGILDSEFSFQASSVQFLNQYGFNYNKFLKNGIPYMNEEQEKKIRHDILTGNWRVRSSPDKDQIKVVIDEVTRWLELAKEGDWMTLPGITGFQAFEVQLVLRQALPNIWTVLKDEGVVVKKVSKQHRWYLQNTSCDRESCWKENILLSARGFSVFFQMLVKAQKPLVGHNMMMDLLHLHEKFFRPLPESYDQFKQNIHSLFPVLIDTKSVTKDIWKEMNFPRVSNLSEVYEVLNSDLNPTKNSGPEIVHASRCEKYVETKCPHEAAYDAFLCGSVLLKVAHLLLQKIYHIDPVPESSFPQYLDVLAPYVNQVNLIRAGVPKINFSGPDYPSIRPPILILSVKRWPGVSEQQVYHKFQNLCKFDVRRLTRSQFLLLTNKFKDARNILKEYRDHPTLCISLYRYWRHSPNVNCLLQVCGIVTAWALLAFILGRSGT
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Molecular Weight
57.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PNLDC1 (Poly(A)-specific ribonuclease domain-containing protein 1) is a crucial enzyme involved in the regulation of RNA metabolism, particularly in the modulation of polyadenylated mRNA degradation. Its role in the cellular RNA surveillance mechanisms and its impact on gene expression make it a target of interest in various biological studies. The interest in PNLDC1 has surged due to its potential implications in diseases, including cancer and neurodegenerative disorders, where dysregulation of RNA processing often plays a pivotal role. The ability to produce recombinant PNLDC1 protein presents significant opportunities for elucidating its functional mechanisms and interactions with RNA substrates. Investigating this protein's characteristics through recombinant technology can provide insights into its enzymatic activity, substrate specificity, and regulatory pathways. Understanding PNLDC1's structure and function may not only advance our comprehension of RNA biology but could also lead to novel therapeutic strategies that harness the modulation of RNA decay pathways in disease contexts. Consequently, the research into PNLDC1 recombinant protein is crucial for developing targeted interventions and understanding the complex landscape of RNA-mediated regulation within cells.
