Analytical Data
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基因名
PKCe
- Application
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别名
PKCe;PKCE;Protein kinase C epsilon type
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q02156
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表达区间
580-737aa
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氨基酸序列
QELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVW LSKEAVSILKAFMTKNPHKRLGCVASQNGEDAIKQHPFFKEIDWVLLEQK KIKPPFKPRIKTKRDVNNFDQDFTREEPVLTLVDEAIVKQINQEEFKGFS YFGEDLMP
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分子量
18 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PKCe, or Protein Kinase C epsilon, is a member of the protein kinase C family, which plays a critical role in various cellular processes, including signal transduction, cell growth, and differentiation. Research on PKCe has gained significant attention due to its involvement in several physiological and pathological conditions, including cancer, cardiovascular diseases, and neurodegenerative disorders. The unique regulatory mechanisms of PKCe, which react to both diacylglycerol and phosphatidylserine, highlight its importance in transducing signals in response to cellular stimuli. Furthermore, studies have shown that PKCe can exhibit distinct functions depending on its localization within the cell, making it a fascinating target for therapeutic interventions. Understanding the structural and functional properties of recombinant PKCe proteins has led to insights into their role in disease contexts and potential applications in drug development. The development of recombinant PKCe proteins facilitates detailed investigations into enzyme kinetics, substrate specificity, and interaction with other signaling molecules, thereby contributing to the elucidation of complex signaling pathways. This has significant implications in designing selective inhibitors or activators that could modulate PKCe activity, ultimately advancing the treatment strategies for related diseases. As such, research into PKCe and its recombinant forms is crucial for developing targeted therapies that leverage this kinase's unique biological functions.












