Analytical Data
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基因名
Ctcfl
- Application
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别名
Ctcfl；BORIS；Transcriptional repressor CTCFL
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49711
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表达区间
266-727aa
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氨基酸序列
FQCELCSYTCPRRSNLDRHMKSHTDERPHKCHLCGRAFRTVTLLRNHLNTHTGTRPHKCPDCDMAFVTSGELVRHRRYKHTHEKPFKCSMCDYASVEVSKLKRHIRSHTGERPFQCSLCSYASRDTYKLKRHMRTHSGEKPYECYICHARFTQSGTMKMHILQKHTENVAKFHCPHCDTVIARKSDLGVHLRKQHSYIEQGKKCRYCDAVFHERYALIQHQKSHKNEKRFKCDQCDYACRQERHMIMHKRTHTGEKPYACSHCDKTFRQKQLLDMHFKRYHDPNFVPAAFVCSKCGKTFTRRNTMARHADNCAGPDGVEGENGGETKKSKRGRKRKMRSKKEDSSDSENAEPDLDDNEDEEEPAVEIEPEPEPQPVTPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVEVKKEPDAEPAEGEEEEAQPAATDAPNGDLTPEMILSMMDR
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分子量
57.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Ctcfl, also known as BORIS (Brother of the Regulator of Imprinted Sites), is a member of the CTCF (CCCTC-binding factor) family of proteins, playing a significant role in gene regulation and chromatin architecture. Emerging evidence has highlighted its involvement in various biological processes, including genomic imprinting, cell differentiation, and the maintenance of stem cell properties. Ctcfl is notably expressed in germ cells and cancer tissues, suggesting its dual function in normal development and oncogenesis. Studies have shown that Ctcfl can modulate the transcriptional activity of specific genes by remodeling chromatin structures, thus influencing gene expression patterns. Its interaction with long non-coding RNAs and regulatory elements emphasizes its potential as a key regulatory hub in the genome. The reconstitution of Ctcfl in vitro provides valuable insights into its molecular functions and regulatory mechanisms. Given its essential roles in both normal cellular processes and pathological conditions, Ctcfl has attracted considerable interest in cancer research and developmental biology. Understanding the functional dynamics of Ctcfl could reveal novel therapeutic targets and biomarker candidates for various cancers, offering promising avenues for future research and clinical applications.
