Analytical Data
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Gene name
Ctcfl
- Application
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Alternative Names
Ctcfl；BORIS；Transcriptional repressor CTCFL
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49711
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Expression Region
266-727aa
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AA Sequence
FQCELCSYTCPRRSNLDRHMKSHTDERPHKCHLCGRAFRTVTLLRNHLNTHTGTRPHKCPDCDMAFVTSGELVRHRRYKHTHEKPFKCSMCDYASVEVSKLKRHIRSHTGERPFQCSLCSYASRDTYKLKRHMRTHSGEKPYECYICHARFTQSGTMKMHILQKHTENVAKFHCPHCDTVIARKSDLGVHLRKQHSYIEQGKKCRYCDAVFHERYALIQHQKSHKNEKRFKCDQCDYACRQERHMIMHKRTHTGEKPYACSHCDKTFRQKQLLDMHFKRYHDPNFVPAAFVCSKCGKTFTRRNTMARHADNCAGPDGVEGENGGETKKSKRGRKRKMRSKKEDSSDSENAEPDLDDNEDEEEPAVEIEPEPEPQPVTPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVEVKKEPDAEPAEGEEEEAQPAATDAPNGDLTPEMILSMMDR
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Molecular Weight
57.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Ctcfl, also known as BORIS (Brother of the Regulator of Imprinted Sites), is a member of the CTCF (CCCTC-binding factor) family of proteins, playing a significant role in gene regulation and chromatin architecture. Emerging evidence has highlighted its involvement in various biological processes, including genomic imprinting, cell differentiation, and the maintenance of stem cell properties. Ctcfl is notably expressed in germ cells and cancer tissues, suggesting its dual function in normal development and oncogenesis. Studies have shown that Ctcfl can modulate the transcriptional activity of specific genes by remodeling chromatin structures, thus influencing gene expression patterns. Its interaction with long non-coding RNAs and regulatory elements emphasizes its potential as a key regulatory hub in the genome. The reconstitution of Ctcfl in vitro provides valuable insights into its molecular functions and regulatory mechanisms. Given its essential roles in both normal cellular processes and pathological conditions, Ctcfl has attracted considerable interest in cancer research and developmental biology. Understanding the functional dynamics of Ctcfl could reveal novel therapeutic targets and biomarker candidates for various cancers, offering promising avenues for future research and clinical applications.
