Analytical Data
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基因名
TMLHE
- Application
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别名
TMLHE；TMLH；Trimethyllysine dioxygenase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NVH6
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表达区间
16-376aa
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氨基酸序列
LLKGGVIYPALPQPNFKSLLPLAVHWHHTASKSLTCAWQQHEDHFELKYANTVMRFDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTIRLDETTLFFTWPDGHVTKYDLNWLVKNSYEGQKQKVIQPRILWNAEIYQQAQVPSVDCQSFLETNEGLKKFLQNFLLYGIAFVENVPPTQEHTEKLAERISLIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGIQVFHCLKHEGTGGRTLLVDGFYAAEQVLQKAPEEFELLSKVPLKHEYIEDVGECHNHMIGIGPVLNIYPWNKELYLIRLFKEKQNTVNRQWNSSLQCDIPERILTYRHFVSGTSIEHRGSLI
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分子量
46.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TMLHE (Trimethyllysine Hydroxylase) is an enzyme involved in the metabolic pathways of trimethyllysine, an amino acid derivative, which plays a key role in the biosynthesis of carnitine—a molecule essential for fatty acid metabolism in cells. The study of TMLHE and its recombinant forms has gained significant attention due to its implications in metabolic disorders, energy homeostasis, and potential therapeutic applications. Mutations or deficiencies in TMLHE can lead to a reduction in carnitine levels, resulting in various health issues, including muscle weakness, cardiomyopathy, and impaired fatty acid metabolism. By producing recombinant TMLHE, researchers aim to elucidate the enzyme's structure-function relationship, understand its catalytic mechanisms, and explore its role in metabolic diseases. Additionally, recombinant TMLHE can be utilized in the development of diagnostic tools and possible treatments for conditions associated with altered carnitine metabolism. The characterization of this enzyme is critical not only for understanding fundamental biochemical processes but also for advancing potential therapeutic strategies that could address related health complications. Overall, the research surrounding TMLHE and its recombinant protein forms offers promising avenues for improving our comprehension of metabolic pathways and their relevance to human health.
