Analytical Data
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Gene name
yscM
- Application
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Alternative Names
yscM；Yop Proteins translocation Protein M
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P69979
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Expression Region
1-115aa
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AA Sequence
MKINTLQSLINQQITQVGHGGQAGRLTETNPLTENSHQISTAEKAFANEVLEHVKNTALSRHDIACLLPRVSNLELKQGKAGEVIVTGLRTEQLSLSDAKLLLEAAMRQDTAADG
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Molecular Weight
28.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of recombinant YscM proteins has gained significant attention due to their crucial role in the pathogenicity of various bacterial species, particularly within the Yersinia genus, which includes notorious pathogens such as Yersinia pestis. YscM, a member of the Yersinia secreted protein (Ysp) family, functions as a regulatory agent in type III secretion systems (T3SS), which are essential for the virulence of these bacteria. These systems enable the injection of effector proteins directly into host cells, facilitating bacterial survival and evasion of host immune responses. Understanding the molecular mechanisms of YscM and its interactions with host cell pathways can provide insights into the strategies employed by pathogenic bacteria to manipulate host cell functions. Additionally, recombinant YscM proteins serve as valuable tools for vaccine development and therapeutic interventions, offering the potential for novel strategies to combat bacterial infections. By elucidating the structure, function, and regulation of YscM, researchers aim to uncover new therapeutic targets and develop innovative approaches to prevent and treat infections caused by Yersinia and related pathogens. The investigation of recombinant YscM proteins thus stands at the intersection of microbiology, immunology, and biotechnology, highlighting their importance in infectious disease research and public health.
