Analytical Data
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基因名
pbpD
- Application
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别名
pbpD；Penicillin-binding Protein 4
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P40750
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表达区间
213-450aa
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氨基酸序列
PNNPTLYDPLKHFDYTKSRQERLLKGLKDAGVITDKELKKAVKQKIKLDVEKREDKYPDYVSYVNDEFTQLVSESEGFDKRLQKASGKQKEKIENELSARVSTLMKDGVKIYTALDPYMQNQVVAQMNSKLPYADVQGGAAVINHQTHQIIALSGGKNYQKYDFNRAYQAYRQPGSSIKPLLDYGPYIEQTGATTSSTIDASKFCSKDYCPQNYNNRTYGTVTLDTAFKNSYNTPAIR
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分子量
43.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PBP-D, or Penicillin-Binding Protein D, is an essential component in the cell wall biosynthesis of bacteria, particularly in Gram-negative organisms. Its primary role is to facilitate the cross-linking of peptidoglycan, which provides structural integrity and shape to bacterial cells. Research on PBP-D has gained significance due to the rising prevalence of antibiotic-resistant strains of bacteria, making it a vital target for novel antimicrobial drug development. By investigating the structure and function of PBP-D, scientists aim to understand its mechanism of action and interactions with beta-lactam antibiotics, which inhibit cell wall synthesis. Additionally, the recombinant expression of PBP-D offers a platform for studying its enzymatic properties and protein-protein interactions in detail. This research not only contributes to the fundamental understanding of bacterial cell biology but also lays the groundwork for innovative therapeutic strategies against multidrug-resistant infections. Overall, the study of PBP-D represents a critical avenue in microbiology and drug discovery, with the potential to address significant public health challenges posed by resistant bacterial pathogens.
