Analytical Data
-
Gene name
RECQL4
- Application
-
Alternative Names
ATP dependent DNA helicase Q4; ATP-dependent DNA helicase Q4; DNA helicase; DNA helicase. RecQ like type 4; RECQ 4; RECQ L4; RecQ protein 4; RecQ protein like 4; RecQ protein-like 4; RecQ-like type 4; RecQ4; RECQ4_HUMAN; RECQL 4; RECQL4; RTS
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O94761
-
Expression Region
1-652 aa
-
AA Sequence
MTRKQRESVLQKIRAAQVHVLMLTPEALVGAGGLPPAAQLPPVAFACIDEAHCLSQWSHNFRPCYLRVCKVLRERMGVHCFLGLTATATRRTASDVAQHLAVAEEPDLHGPAPVPTNLHLSVSMDRDTDQALLTLLQGKRFQNLDSIIIYCNRREDTERIAALLRTCLHAAWVPGSGGRAPKTTAEAYHAGMCSRERRRVQRAFMQGQLRVVVATVAFGMGLDRPDVRAVLHLGLPPSFESYVQAVGRAGRDGQPAHCHLFLQPQGEDLRELRRHVHADSTDFLAVKRLVQRVFPACTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSHQAAPGPRRVCMGHERALPIQLTVQALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRLNCPGGPAQLQALAHRCPPLAVCLAQQLPEDPGQGSSSVEFDMVKLVDSMGWELASVRRALCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQICDFLYGRVQARERQALARLRRTFQAFHSVAFPSCGPCLEQQDEERSTRLKDLLGRYFEEEEGQEPGGMEDAQGPEPGQARLQDWEDQVRCDIRQFLSLRPEEKFSSRAVARIFHGIGSPCYPAQVYGQDRRFWRKYLHLSFHALVGLATEELLQVAR
-
Molecular Weight
97.46 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RECQL4 is a member of the RecQ helicase family, which plays a crucial role in maintaining genomic stability by resolving DNA structures during replication and repair. Mutations in the RECQL4 gene are associated with various genetic disorders, including Rothmund-Thomson syndrome, which is characterized by childhood skin rashes, skeletal abnormalities, and a predisposition to cancer. Research into RECQL4 has gained significant attention due to its involvement in critical cellular processes such as DNA damage response and telomere maintenance. Understanding the function and mechanisms of RECQL4 is essential for elucidating its role in these disorders and for exploring potential therapeutic strategies. Recent studies utilizing recombinant protein techniques have focused on characterizing the biochemical properties of RECQL4, including its helicase activity, interactions with other proteins, and its response to DNA damage. By elucidating these mechanisms, researchers aim to advance our understanding of the relationship between RECQL4 and genomic stability, ultimately contributing to the development of targeted interventions for diseases linked to RECQL4 dysfunction. This research not only enhances our knowledge in the field of molecular genetics but also lays the groundwork for innovative approaches to cancer treatment and prevention.
