Analytical Data
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基因名
EDEM2
- Application
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别名
EDEM2；C20orf31；C20orf49；ER degradation-enhancing alpha-mannosidase-like Protein 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BV94
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表达区间
1-492aa
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氨基酸序列
MPFRLLIPLG LLCALLPQHH GAPGPDGSAP DPAHYRERVK AMFYHAYDSY LENAFPFDEL RPLTCDGHD TWGSFSLTLI DALDTLLILG NVSEFQRVVE VLQDSVDFDI DVNASVFETN IRVVGGLLS AHLLSKKAGV EVEAGWPCSG PLLRMAEEAA RKLLPAFQTP TGMPYGTVNL LHGVNPGET PVTCTAGIGT FIVEFATLSS LTGDPVFEDV ARVALMRLWE SRSDIGLVGN HIDVLTGKW VAQDAGIGAG VDSYFEYLVK GAILLQDKKL MAMFLEYNKA IRNYTRFDDW YLWVQMYKG TVSMPVFQSL EAYWPGLQSL IGDIDNAMRT FLNYYTVWKQ FGGLPEFYNI PQGYTVEKR EGYPLRPELI ESAMYLYRAT GDPTLLELGR DAVESIEKIS KVECGFATIK DLRDHKLDN RMESFFLAET VKYLYLLFDP TNFIHNNGST FDAVITPYGE CILGAGGYIF NTEAHPIDP AALHCCQRLK
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分子量
54 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EDEM2 (ER degradation-enhancing alpha-mannosidase like protein 2) is an essential component of the endoplasmic reticulum-associated degradation (ERAD) pathway, which plays a crucial role in cellular quality control by facilitating the degradation of misfolded glycoproteins. With the increasing understanding of protein folding and degradation mechanisms in the endoplasmic reticulum, EDEM2 has garnered significant research interest. Its primary function is to bind to misfolded glycoproteins, promoting their retrotranslocation from the ER to the cytosol, where they can be ubiquitinated and subsequently degraded by the proteasome. Dysregulation of EDEM2 and the ERAD pathway has been implicated in various diseases, including neurodegenerative disorders and certain cancers, which highlights its potential as a therapeutic target. Recent studies have focused on elucidating the molecular mechanisms by which EDEM2 recognizes and processes misfolded proteins, as well as its interactions with other ERAD components. Additionally, research involving the recombinant expression of EDEM2 has facilitated the investigation of its functional properties and its role in maintaining protein homeostasis within the cell. Understanding the mechanisms of EDEM2 can provide insight into the broader implications of ER stress and protein misfolding in health and disease, paving the way for the development of novel therapeutic strategies aimed at enhancing ER quality control mechanisms.
