Analytical Data
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基因名
phrB
- Application
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别名
phrB；(6-4) photolyase
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P00914
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表达区间
1-472aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSMTTHLVWFRQDLRLHDNLALAAACRNS SARVLALYIATPRQWATHNMSPRQAELINAQLNGLQIALAEKGIPLLFRE VDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVEVERALRNVVCE GFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPKVRS SGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQNGAGEYEQQR DFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNELI WREFYRHLITYHPSLCKHRPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIV DAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLA ANNGGWQWAASTGTDAAPYFRIFNPTTQGEKFDHEGEFIRQWLPELRDVP GKVVHEPWKWAQKAGVTLDYPQPIVEHKEARVQTLAAYEAARKGK
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分子量
56 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of the phrB recombinant protein is rooted in its significant role in bacterial communication and environmental adaptation. PhrB is a part of the quorum sensing system in certain bacteria, particularly in species like *Bacillus subtilis*, where it functions as a signaling molecule that regulates gene expression in response to population density. Understanding the structure and function of phrB can provide insights into the mechanisms of bacterial behavior, such as biofilm formation, virulence, and antibiotic resistance. The recombinant production of phrB enables researchers to investigate its biochemical properties and interactions with other cellular components in a controlled laboratory setting. Such studies can reveal how phrB contributes to bacterial survival and adaptability under various stress conditions, offering potential applications in biotechnology and medicine. For instance, modulating the activity of phrB or its pathways may lead to novel strategies for managing bacterial infections or enhancing the efficacy of probiotics. Overall, the investigation of phrB recombinant protein stands at the intersection of microbiology, genetics, and therapeutic development, highlighting its relevance in both fundamental and applied sciences.
