Analytical Data
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基因名
RNF25
- Application
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别名
AO7; E3 ubiquitin protein ligase RNF25; E3 ubiquitin-protein ligase RNF25; EC 6.3.2.-; FLJ13906; RING finger protein 25; RING finger protein AO7; RNF 25; RNF25; RNF25_HUMAN
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96BH1
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表达区间
1-459 aa
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氨基酸序列
MAASASAAAG EEDWVLPSEV EVLESIYLDE LQVIKGNGRT SPWEIYITLH PATAEDQDSQ YVCFTLVLQV PAEYPHEVPQ ISIRNPRGLS DEQIHTILQV LGHVAKAGLG TAMLYELIEK GKEILTDNNI PHGQCVICLY GFQEKEAFTK TPCYHYFHCH CLARYIQHME QELKAQGQEQ EQERQHATTK QKAVGVQCPV CREPLVYDLA SLKAAPEPQQ PMELYQPSAE SLRQQEERKR LYQRQQERGG IIDLEAERNR YFISLQQPPA PAEPESAVDV SKGSQPPSTL AAELSTSPAV QSTLPPPLPV ATQHICEKIP GTRSNQQRLG ETQKAMLDPP KPSRGPWRQP ERRHPKGGEC HAPKGTRDTQ ELPPPEGPLK EPMDLKPEPH SQGVEGPPQE KGPGSWQGPP PRRTRDCVRW ERSKGRTPGS SYPRLPRGQG AYRPGTRRES LGLESKDGS
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分子量
51.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF25, a member of the RING finger E3 ubiquitin ligase family, has emerged as a crucial player in various cellular processes, including protein degradation, signal transduction, and gene regulation. Its unique RING finger motif facilitates the transfer of ubiquitin to target proteins, influencing their stability and activity. Recent studies have highlighted RNF25's involvement in tumorigenesis, where it can modulate pathways related to cancer cell proliferation and apoptosis. Additionally, RNF25 has been linked to the regulation of immune responses, suggesting a broader role in maintaining cellular homeostasis. Given its pivotal functions, the production of recombinant RNF25 has become essential for detailed mechanistic studies, enabling researchers to explore its interactions, substrate specificity, and potential as a therapeutic target. Understanding RNF25's role in disease contexts is of paramount importance, as it could lead to novel strategies for treating various conditions, particularly cancers where its dysregulation is evident. Therefore, the study of RNF25 recombinant protein not only provides insights into its biological significance but also paves the way for future translational research aimed at leveraging this E3 ligase in clinical applications.
