Analytical Data
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基因名
LEPR
- Application
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别名
LEPR；DB；OBR；Leptin receptor
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P48357
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表达区间
22-839aa
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氨基酸序列
FNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPK FNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVFQ QIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLED SPLVPQKGSFQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVI FQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQV KYSENSTTVIREADKIVSATSLLVDSILPGSSYEVQVRGKRLDGPGIWSD WSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMN LAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHHR YAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSL YCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGS LDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQ IRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGL GYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMK NDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINS IGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMY FIIEWKNLNEDGEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGV GKPKIINSFTQDDIEKHQSD
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分子量
94 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Leptin is a critical hormone involved in regulating energy balance and body weight, primarily produced by adipocytes. Its mechanism of action is mediated by the leptin receptor (LEPR), which is a member of the class I cytokine receptor family. Variations in LEPR signaling can lead to obesity and metabolic disorders, as impaired leptin function diminishes the body's ability to regulate hunger and energy expenditure. Recombinant LEPR protein studies have emerged as a valuable tool to elucidate the receptor's structure, function, and its complex signaling pathways. By employing recombinant DNA technology, researchers can produce large quantities of LEPR, allowing for detailed biochemical and biophysical characterization. These studies aim to identify the receptor’s interactions with various ligands and downstream signaling molecules, which are crucial for understanding how LEPR dysfunction contributes to obesity and its related conditions. The generation of LEPR mutants also facilitates investigations into the effects of specific genetic variations on receptor functionality. Overall, research involving LEPR recombinant proteins holds significant promise for developing therapeutic strategies targeting leptin signaling to combat obesity and metabolic diseases.
