Analytical Data
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Gene name
SEPT14
- Application
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Alternative Names
SEPTIN14; SEPT14; Septin-14
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A6QQL3
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Expression Region
1-432 aa
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AA Sequence
MAEKPMCPST QIPDDEDTQK EDNIRCLNML GHFGFDCLAH QLVDKSVQQG FFFNILCVGE TGIGKSTLID TLFNTSLKDK KSSHFYASVG LKIQTYELQE NNVQLKLTVV KTVGYGDQIN KEASYQPIVD YLDAQFESYL QEELKIKRSL GNYRDSRVHV CLYFISPTGH SLKSLDILTM KNIDSKVNII PVIAKADAIS KSDLQTFKCA IMNELISNGI QMYQFPTDNE TSTHMNSSMN GLLPFAVVGS TEEVKVGKRT VRGRQYPWGI LQVENENHCD FVKLRDMLLC TNREDLKEQT HTRHYERYRR NRLHMMGFTD MGPNNQPVSF QEIYEAKRQE LLEQCQREEE ELKHKFMQRV KEKETAFKEA EKELQDKFEH LKKVQQEETM RLEEERRQLE EEILELCKMK ASSGTLQSQV CTSVKKDKER KK
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Molecular Weight
50.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SEPT14, a member of the septin protein family, plays a crucial role in various cellular processes, including cytokinesis, membrane dynamics, and the formation of cytoskeletal structures. The interest in SEPT14 has grown due to its potential implications in reproductive biology, particularly in male fertility, where it is involved in the differentiation of sperm cells and the integrity of sperm motility. Studies have indicated that mutations or aberrant expression of SEPT14 can lead to infertility in males, highlighting its essential function in spermatogenesis. Furthermore, SEPT14's unique structural properties and its capability to form hetero-oligomeric complexes have attracted attention for its potential as a therapeutic target. Research into the mechanisms by which SEPT14 interacts with other proteins and its role in cellular signaling pathways is ongoing, with the aim of unveiling its functions in health and disease. Understanding SEPT14’s structure and function could not only provide insights into fundamental biological processes but also aid in the development of strategies for addressing male infertility and other related disorders. The reconstitution of SEPT14 proteins in studies aims to elucidate their behavior in vitro, facilitating a deeper understanding of their roles and the impact of mutations on their function. This research stands to enhance our knowledge of septin biology and its significance in reproductive health, thereby informing future clinical approaches.
