Analytical Data
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Gene name
SBP65
- Application
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Alternative Names
SBP65；Seed biotin-containing Protein SBP65
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q39846
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Expression Region
1-255aa
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AA Sequence
MASEQLARRENTTTEKEIHVEKHRVPKMATHFEHLAEQAKESDITAGKDTPQGSIEALQAGERVKDHAGKAMGDIGGRGKARETHELGAHFESLADKVTDHAAANVVGNKESQREARGGVRDVGKFEMRTEGGEKGNKDRPELKTRTREVIGRTEKERGRESGGQVVAEKGRETETARGRVGAENEGARTTAVITCTLEKGGGTQKPIREEERESESERSAWEQISNYSDQATQGVKEKYERAKQAASETLNTTT
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Molecular Weight
34.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SBP65 is a recombinant protein derived from the SBP (Streptavidin-Binding Peptide) that has garnered significant interest in the field of biotechnology and molecular biology. The SBP, originally isolated from *Streptomyces avidinii*, exhibits a strong affinity for biotin, making it an invaluable tool in various applications such as protein labeling, purification, and detection. Researchers have been increasingly exploring SBP65 due to its ability to serve as a versatile scaffold for protein engineering, enabling the development of biosensors and diagnostic tools. The recombinant production of SBP65 allows for the generation of large quantities, facilitating its use in high-throughput systems. Recent studies have focused on optimizing its expression and purification protocols, as well as enhancing its binding properties through mutagenesis. Furthermore, understanding the structural and functional aspects of SBP65 is pivotal for its application in biotechnological innovations, including targeted drug delivery and the creation of biotinylated fusion proteins. As research progresses, the applications of SBP65 could expand, potentially impacting areas such as therapeutics, vaccine development, and the enhancement of scientific research methodologies. Overall, the study of SBP65 represents a significant convergence of protein engineering and molecular recognition, promising to contribute to advances in medical and biological research.
