Analytical Data
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基因名
dnapkcs
- Application
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别名
dnapkcs；HYRC；HYRC1；DNA-dependent Protein kinase catalytic subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P78527
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表达区间
3746-4128aa
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氨基酸序列
REHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSACSQRALQLRTYSVVP MTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYKDWLTK MSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPE AFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHA FGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDP GLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKIC YAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPE SGLSE ETQVKCLMDQATDPNILGRTWEGWEPWM
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分子量
68 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNA-dependent protein kinase (DNA-PK) is a crucial enzyme involved in the non-homologous end joining (NHEJ) pathway of DNA double-strand break repair. The research on DNA-PK and its recombinant protein has garnered significant attention due to its role in maintaining genomic stability and its implications in cancer biology. DNA-PK consists of a catalytic subunit (DNA-PKcs) and a regulatory component (KU70/KU80 heterodimer), which recognize and bind to broken DNA ends, facilitating the recruitment of additional repair factors. Understanding the structure and function of DNA-PKcs is essential for elucidating the mechanisms of DNA repair and the cellular response to genotoxic stress. Furthermore, aberrations in DNA-PK activity have been linked to various malignancies, making it an attractive target for therapeutic interventions. Recent advancements in recombinant protein technology have enabled the production and purification of DNA-PKcs, allowing researchers to study its biochemical properties, regulatory mechanisms, and interactions with other DNA repair proteins. Insights gained from these studies may lead to the development of novel cancer therapies that enhance the efficacy of radiotherapy and chemotherapy by targeting DNA repair pathways. Thus, the investigation of DNA-PKcs as a recombinant protein is crucial for advancing our understanding of DNA repair mechanisms and providing potential avenues for cancer treatment.
