Analytical Data
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Gene name
SH2B2
- Application
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Alternative Names
SH2B2; APS; SH2B adapter protein 2; Adapter protein with pleckstrin homology and Src homology 2 domains; SH2 and PH domain-containing adapter protein APS
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14492
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Expression Region
1-632 aa
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AA Sequence
MNGAGPGPAA AAPVPVPVPV PDWRQFCELH AQAAAVDFAH KFCRFLRDNP AYDTPDAGAS FSRHFAANFL DVFGEEVRRV LVAGPTTRGA AVSAEAMEPE LADTSALKAA PYGHSRSSED VSTHAATKAR VRKGFSLRNM SLCVVDGVRD MWHRRASPEP DAAAAPRTAE PRDKWTRRLR LSRTLAAKVE LVDIQREGAL RFMVADDAAA GSGGSAQWQK CRLLLRRAVA EERFRLEFFV PPKASRPKVS IPLSAIIEVR TTMPLEMPEK DNTFVLKVEN GAEYILETID SLQKHSWVAD IQGCVDPGDS EEDTELSCTR GGCLASRVAS CSCELLTDAV DLPRPPETTA VGAVVTAPHS RGRDAVRESL IHVPLETFLQ TLESPGGSGS DSNNTGEQGA ETDPEAEPEL ELSDYPWFHG TLSRVKAAQL VLAGGPRNHG LFVIRQSETR PGEYVLTFNF QGKAKHLRLS LNGHGQCHVQ HLWFQSVLDM LRHFHTHPIP LESGGSADIT LRSYVRAQDP PPEPGPTPPA APASPACWSD SPGQHYFSSL AAAACPPASP SDAAGASSSS ASSSSAASGP APPRPVEGQL SARSRSNSAE RLLEAVAATA AEEPPEAAPG RARAVENQYS FY
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Molecular Weight
67.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SH2B2, also known as SH2 domain-containing protein B, is a crucial adaptor protein that plays a significant role in various cellular signaling pathways, particularly those related to insulin receptor signaling and immune responses. As a member of the SH2B family, SH2B2 is characterized by its SH2 domain, which enables it to interact with phosphorylated tyrosine residues on activated receptors and other signaling proteins. Over the years, research has revealed that SH2B2 is involved in the regulation of cytokine signaling, neuronal development, and metabolic processes. Dysregulation of SH2B2 has been associated with several pathological conditions, including obesity, type 2 diabetes, and autoimmune diseases. Consequently, understanding the structure and function of SH2B2 has garnered considerable interest in the fields of molecular biology and biochemistry. Researchers have focused on the recombinant expression of SH2B2 to investigate its biochemical properties, interaction dynamics, and functional significance in signaling cascades. Recombinant SH2B2 proteins facilitate the elucidation of SH2B2's role in cellular processes and its potential as a therapeutic target. By employing various techniques such as site-directed mutagenesis and protein-protein interaction assays, scientists aim to characterize the mechanisms underlying SH2B2's functions and its contributions to disease. This knowledge not only enhances our understanding of cellular signaling but also paves the way for the development of novel strategies to modulate SH2B2 activity in therapeutic contexts.
