Analytical Data
-
基因名
HIST3H2A
- Application
-
别名
HIST3H2A；H2AW；HIST3H2A；Histone H2A type 3
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q7L7L0
-
表达区间
2-130aa
-
氨基酸序列
SGRGKQGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGNY SERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGR VTIAQGGVLP NIQAVLLPKK TESHHKAKGK
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HIST3H2A is a histone protein that plays a crucial role in the structure and function of chromatin, which is essential for regulating gene expression and maintaining genomic integrity. As a key component of the nucleosome, HIST3H2A participates in the packaging of DNA into a compact form, influencing various cellular processes such as DNA replication, repair, and transcription. Understanding the molecular mechanisms involving HIST3H2A is vital for deciphering the complex regulation of gene expression and the implications of histone modification in diseases, including cancer and genetic disorders. The study of recombinant HIST3H2A proteins allows researchers to investigate their biochemical properties, interactions with DNA and other proteins, and the effects of post-translational modifications. Moreover, recombinant histones can be used to create versatile in vitro models for exploring chromatin dynamics and the impact of histone variants on cellular functions. This research not only enhances our understanding of epigenetic regulation but also opens avenues for potential therapeutic strategies targeting chromatin-related pathways. Overall, the investigation of HIST3H2A and its recombinant forms is a critical area of research in molecular biology, with significant implications for genetics, epigenetics, and disease treatment.
