Analytical Data
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Gene name
GRIA4
- Application
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Alternative Names
GRIA4；GluA4；GLUR4；Glutamate receptor 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48058-2
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Expression Region
1-433aa
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AA Sequence
MRIISRQIVLLFSGFWGLAMGAFPSSVQIGGLFIRNTDQEYTAFRLAIFL HNTSPNASEAPFNLVPHVDNIETANSFAVTNAFCSQYSRGVFAIFGLYDK RSVHTLTSFCSALHISLITPSFPTEGESQFVLQLRPSLRGALLSLLDHYE WNCFVFLYDTDRGYSILQAIMEKAGQNGWHVSAICVENFNDVSYRQLLEE LDRRQEKKFVIDCEIERLQNILEQIVSVGKHVKGYHYIIANLGFKDISLE RFIHGGANVTGFQLVDFNTPMVIKLMDRWKKLDQREYPGSETPPKYTSAL TYDGVLVMAETFRSLRRQKIDISRRGNAGDCLANPAAPWGQGIDMERTLK QVRIQGLTGNVQFDHYGRRVNYTMDVFELKSTGPRKVGYWNDMDKLVLIQ DVPTLGNDTAAIENRTVVVTTIMPLMKNPILRN
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Molecular Weight
76 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GRIA4, a member of the glutamate receptor ion channel family, plays a crucial role in synaptic transmission and plasticity in the central nervous system. This protein is predominantly expressed in the brain and is involved in mediating excitatory neurotransmission, contributing to various physiological processes including learning and memory. Research has identified GRIA4's significance in neurodevelopmental disorders and neurodegenerative diseases, highlighting its potential as a therapeutic target. However, the structural and functional characterization of GRIA4 has remained limited due to challenges in obtaining sufficient quantities of the recombinant protein. To overcome these barriers, efforts have been directed towards the successful expression and purification of GRIA4 in a heterologous system. Understanding the molecular mechanisms underlying GRIA4's function, along with the development of specific modulators, could pave the way for innovative treatment strategies for various brain disorders. This research aims not only to elucidate the functional properties of GRIA4 but also to explore its interactions with other proteins and ligands, providing insights into its regulatory mechanisms and potential implications in medical science.
