Analytical Data
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基因名
CYP39A1
- Application
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别名
24 hydroxycholesterol 7 alpha hydroxylase; 24-hydroxycholesterol 7-alpha-hydroxylase; CP39A_HUMAN; Cyp39a1; cytochrome P450 family 39; subfamily A polypeptide 1; Cytochrome P450 39A1; cytochrome P450 subfamily XXXIX (oxysterol 7 alpha hydroxylase) polypeptide 1
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NYL5
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表达区间
1-469aa
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氨基酸序列
MELISPTVIIILGCLALFLLLQPKNLRRPPCIKGWIPWIGVGFEFGKAPLEFIEKARIKYGPIFTVFAMGNRMTFVTEEEGINVFLKSKKVDFELAVQNIVYRTASIPKNVFLALHEKLYIMLKGKMGTVNLHQFTGQLTEELHEQLENLGTHGTMDLNNLVRHLLYPVTVNMLFNKSLFSTNKKKIKEFHQYFQVYDEDFEYGSQLPECLLRNWSKSKKWFLELFEKNIPDIKACKSAKDNSMTLLQATLDIVETETSKENSPNYGLLLLWASLSNAVPVAFWTLAHVLSHPDIHKAIMEGISSVFGKAGKDKIKVSEDDLENLLLIKWCVLETIRLKAPGVITRKVVKPVEILNYIIPSGDLLMLSPFWLHRNPKYFPEPELFKPERWKKANLEKHSFLDCFMAFGSGKFQCPARWFALLEVQMCIILILYKYDCSLLDPLPKQSYLHLVGVPQPEGQCRIEYKQRI
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分子量
77.33 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CYP39A1, a member of the cytochrome P450 family, is an enzyme primarily involved in cholesterol metabolism and the biotransformation of sterols. It plays a critical role in the regulation of bile acid synthesis by converting cholesterol into bile acids, thus impacting lipid homeostasis and cardiovascular health. Recent studies have revealed that CYP39A1 not only influences cholesterol levels but also participates in the oxidative metabolism of various xenobiotics, showcasing its potential role in drug metabolism and detoxification processes. The investigation of CYP39A1's recombinant protein is pivotal for understanding its structural and functional characteristics, which may further elucidate its mechanisms of action in sterol metabolism and its implications in metabolic disorders, such as atherosclerosis and non-alcoholic fatty liver disease. By expressing and characterizing CYP39A1, researchers aim to explore its enzymatic activity, substrate specificity, and interaction with other metabolic pathways. Furthermore, the availability of recombinant CYP39A1 may facilitate the development of novel therapeutic strategies targeting cholesterol-related diseases and enhance our understanding of P450 enzyme dynamics in human health.
