Analytical Data
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Gene name
CREB
- Application
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Alternative Names
CREB；Cyclic AMP-responsive element-binding Protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P16220
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Expression Region
1-341aa
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AA Sequence
MTMESGAENQ QSGDAAVTEA ENQQMTVQAQ PQIATLAQVS MPAAHATSSA PTVTLVQLPN GQTVQVHGVI QAAQPSVIQS PQVQTVQSSC KDLKRLFSGT QISTIAESED SQESVDSVTD SQKRREILSR RPSYRKILND LSSDAPGVPR IEEEKSEEET SAPAITTVTV PTPIYQTSSG QYIAITQGGA IQLANNGTDG VQGLQTLTMT NAAATQPGTT ILQYAQTTDG QQILVPSNQV VVQAASGDVQ TYQIRTAPTS TIAPGVVMAS SPALPTQPAE EAARKREVRL MKNREAAREC RRKKKEYVKC LENRVAVLEN QNKTLIEELK ALKDLYCHKS D
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CREB (cAMP Response Element-Binding protein) is a transcription factor that plays a crucial role in regulating gene expression in response to various extracellular signals, particularly those mediated by cyclic AMP (cAMP). It is involved in numerous physiological processes, including energy metabolism, neuroplasticity, and cell survival. The CREB protein functions by binding to specific DNA sequences known as cAMP response elements, facilitating the transcription of target genes associated with learning, memory, and stress responses. Dysregulation of CREB has been implicated in several pathologies, such as depression, addiction, and neurodegenerative diseases. Consequently, understanding the structure and function of CREB, particularly through recombinant protein studies, has become a focal point of research. Recombinant CREB proteins, produced through advanced genetic engineering techniques, allow scientists to dissect the molecular mechanisms underlying CREB's activity, elucidate its interactions with other proteins and nucleic acids, and assess the effects of mutations or post-translational modifications. This research not only enhances our comprehension of CREB's role in cell signaling pathways but also holds potential for therapeutic strategies aimed at modulating CREB activity for the treatment of related disorders.
