Analytical Data
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基因名
DAZ3
- Application
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别名
DAZ3Deleted in azoospermia protein 3
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NR90
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表达区间
1-486aa
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氨基酸序列
MSAANPETPN STISREASTQ SSSAAASQGW VLPEGKIVPN TVFVGGIDAR MDETEIGSCF GRYGSVKEVK IITNRTGVSK GYGFVSFVND VDVQKIVGSQ IHFHGKKLKL GPAIRKQKLC ARHVQPRPLV VNPPPPPQFQ NVWRNPNTET YLQPQITPNP VTQHVQAYSA YPHSPGQVIT GCQLLVYNYQ EYPTYPDSAF QVTTGYQLPV YNYQPFPAYP RSPFQVTAGY QLPVYNYQAF PAYPNSPFQV ATGYQFPVYN YQPFPAYPSS PFQVTAGYQL PVYNYQAFPA YPNSPFQVAT GYQFPVYNYQ AFPAYPNSPV QVTTGYQLPV YNYQAFPAYP NSPFQVATGY QFPVYNYQAF PAYPNSPVQV TTGYQLPVYN YQAFPAYPNS PFQVATGYQF PVYNYQAFPA YPNSPVQVTT GYQLPVYNYQ AFPAYPNSAV QVTTGYQFHV YNYQMPPQCP VGEQRRNLWT EAYKWWYLVC LIQRRD
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分子量
54.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DAZ3 (Deleted in Azoospermia 3) is a member of the DAZ gene family, which is crucial for male fertility. This family of genes is located on the Y chromosome and plays a pivotal role in spermatogenesis. Research surrounding DAZ3 has gained prominence due to its implications in reproductive biology and genetic disorders associated with male infertility, particularly azoospermia. Azoospermia, characterized by the absence of viable sperm in ejaculate, can be linked to deletions or mutations in genes such as DAZ3. Studies have shown that DAZ3 is involved in the regulation of germ cell development and the maintenance of spermatogonial stem cells. As infertility affects a significant portion of the global population, understanding the molecular mechanisms by which DAZ3 operates can provide insights into new diagnostic and therapeutic avenues. Furthermore, DAZ3's evolutionary preservation across species suggests its fundamental role in reproductive processes, making it an important subject for genetic and developmental research. Insights gained from studying DAZ3 may also extend to broader implications in reproductive health and the development of assisted reproductive technologies. Overall, DAZ3 represents a critical area of research for elucidating male fertility dynamics and addressing infertility challenges in clinical settings.
