Analytical Data
-
基因名
ND5
- Application
-
别名
ND5；MTND5；NADH5；ND5；NADH-ubiquinone oxidoreductase chain 5
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P03915
-
表达区间
1-603aa
-
氨基酸序列
MTMHTTMTTLTLTSLIPPILTTLVNPNKKNSYPHYVKSIVASTFIISLFPTTMFMCLDQEVIISNWHWATTQTTQLSLSFKLDYFSMMFIPVALFVTWSIMEFSLWYMNSDPNINQFFKYLLIFLITMLILVTANNLFQLFIGWEGVGIMSFLLISWWYARADANTAAIQAILYNRIGDIGFILALAWFILHSNSWDPQQMALLNANPSLTPLLGLLLAAAGKSAQLGLHPWLPSAMEGPTPVSALLHSSTMVVAGIFLLIRFHPLAENSPLIQTLTLCLGAITTLFAAVCALTQNDIKKIVAFSTSSQLGLMMVTIGINQPHLAFLHICTHAFFKAMLFMCSGSIIHNLNNEQDIRKMGGLLKTMPLTSTSLTIGSLALAGMPFLTGFYSKDHIIETANMSYTNAWALSITLIATSLTSAYSTRMILLTLTGQPRFPTLTNINENNPTLLNPIKRLAAGSLFAGFLITNNISPASPFQTTIPLYLKLTALAVTFLGLLTALDLNYLTNKLKMKSPLCTFYFSNMLGFYPSITHRTIPYLGLLTSQNLPLLLLDLTWLEKLLPKTISQHQISTSIITSTQKGMIKLYFLSFFFPLILTLLLIT
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ND5, a key subunit of the mitochondrial NADH dehydrogenase complex I, plays a crucial role in the electron transport chain, facilitating ATP production in eukaryotic cells. Its importance in cellular metabolism and energy homeostasis makes it a significant focus of research, particularly concerning mitochondrial diseases and metabolic disorders. Mutations in the ND5 gene have been linked to various pathologies, including Leber's hereditary optic neuropathy and other mitochondrial myopathies, underscoring the necessity for detailed studies on this protein. Recent advancements in molecular biology techniques, such as recombinant DNA technology and protein expression systems, have enabled researchers to produce ND5 in vitro, allowing for comprehensive functional analyses. Understanding the structural and functional dynamics of ND5 through recombinant protein studies can provide insights into its role in respiratory chain disorders, paving the way for potential therapeutic interventions. Furthermore, investigating the interactions between ND5 and other mitochondrial proteins may reveal mechanisms of regulation and signaling that are fundamental to mitochondrial function. Overall, ND5 recombinant protein research holds promise for elucidating the complexities of mitochondrial biology and addressing the challenges posed by related diseases.
