Analytical Data
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Gene name
DDX18
- Application
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Alternative Names
ATP dependent RNA helicase DDX18; ATP-dependent RNA helicase DDX18; DDX 18; DDX18; DDX18_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 18; DEAD box polypeptide 18; DEAD box protein 18; FLJ33908; MrDb; Myc regulated DEAD box protein; Myc-regulated DEAD box protein
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NVP1
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Expression Region
1-670aa
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AA Sequence
MSHLPMKLLRKKIEKRNLKLRQRNLKFQGASNLTLSETQNGDVSEETMGSRKVKKSKQKPMNVGLSETQNGGMSQEAVGNIKVTKSPQKSTVLTNGEAAMQSSNSESKKKKKKKRKMVNDAEPDTKKAKTENKGKSEEESAETTKETENNVEKPDNDEDESEVPSLPLGLTGAFEDTSFASLCNLVNENTLKAIKEMGFTNMTEIQHKSIRPLLEGRDLLAAAKTGSGKTLAFLIPAVELIVKLRFMTRNGTGVLILSPTRELAMQTFGVLKELMTHHVHTYGLIMGGSNRSAEAQKLGNGINIIVATPGRLLDHMQNTPGFMYKNLQCLVIDEADRILDVGFEEELKQIIKLLPTRRQTMLFSATQTRKVEDLARISLKKEPLYVGVDDDKANATVDGLEQGYVVCPSEKRFLLLFTFLKKNRKKKLMVFFSSCMSVKYHYELLNYIDLPVLAIHGKQKQNKRTTTFFQFCNADSGTLLCTDVAARGLDIPEVDWIVQYDPPDDPKEYIHRVGRTARGLNGRGHALLILRPEELGFLRYLKQSKVPLSEFDFSWSKISDIQSQLEKLIEKNYFLHKSAQEAYKSYIRAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVNSNEGKQKKRGGGGGFGYQKTKKVEKSKIFKHISKKSSDSRQFSH
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Molecular Weight
101.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DDX18, a member of the DEAD-box RNA helicase family, plays a crucial role in various cellular processes, including RNA metabolism, ribosome biogenesis, and stress responses. Its ATP-dependent unwinding activity is essential for the regulation of RNA structure and function, influencing gene expression and cellular signaling. Research indicates that DDX18 is involved in the assembly and maturation of ribonucleoprotein complexes, highlighting its significance in protein synthesis and cellular homeostasis. Furthermore, dysregulation of DDX18 has been associated with several diseases, including cancers and genetic disorders, making it a potential therapeutic target. The production of recombinant DDX18 protein allows for in-depth studies of its biochemical properties, structural functions, and interactions with RNA and other proteins. This research could provide insights into its role in disease mechanisms and pave the way for the development of novel therapeutic strategies. Overall, investigating DDX18 through recombinant protein studies underscores its importance in cellular functions and its potential as a biomarker or drug target in human health.
