Cat: PA2000-7067

Recombinant Human DEGS1 Protein,GST

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Analytical Data

  • Gene name

    DEGS1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    DEGS1; DES1; MLD; MIG15; Sphingolipid delta(4-desaturase DES1; Cell migration-inducing gene 15 protein; Degenerative spermatocyte homolog 1; Dihydroceramide desaturase-1; Membrane lipid desaturase

  • Species

    Human

  • Source

    E. coli

  • Tag

    GST-tag at N-terminal

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    O15121

  • Expression Region

    1-323aa

  • AA Sequence

    MGSRVSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQKGEMVLE

  • Molecular Weight

    64.3 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

The study of DEGS1 (Delta-5, 4-desaturase, ER localized protein 1) has gained significance due to its crucial role in lipid metabolism, particularly in the synthesis of sphingolipids and the regulation of membrane dynamics. DEGS1 is an integral enzyme located in the endoplasmic reticulum that catalyzes the conversion of dihydroceramide to sphingomyelin, a vital component of cellular membranes, influencing various cellular processes including signal transduction, cell differentiation, and apoptosis. Aberrant DEGS1 function has been linked to several pathological conditions, such as neurodegenerative diseases and cancer, making it a potential therapeutic target. Recent advances in recombinant protein technology have enabled the production of DEGS1, facilitating in-depth studies of its enzymatic activity, structure-function relationships, and interaction with other cellular components. Understanding the molecular mechanisms by which DEGS1 operates can lead to potential interventions in diseases characterized by lipid metabolism dysregulation, thus highlighting the relevance of DEGS1 research within the broader context of biomedical science.

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