Analytical Data
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基因名
POLG2
- Application
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别名
POLG2；MTPOLB；DNA polymerase subunit gamma-2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UHN1
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表达区间
1-485aa
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氨基酸序列
MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPGYLETMQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV
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分子量
70.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLG2, the regulatory subunit of the mitochondrial DNA polymerase, plays a crucial role in mitochondrial DNA replication and repair. It forms a heterotrimer with the catalytic subunit POLG, ensuring the proper functioning of mitochondrial replication machinery. Mutations in the POLG2 gene are linked to various mitochondrial disorders, which often present with neurological symptoms and multisystemic involvement. Research on POLG2 recombinant protein has gained traction due to its potential implications for understanding mitochondrial diseases and developing therapeutic strategies. By producing recombinant POLG2 in suitable expression systems, scientists can study its structural and functional properties, investigate its interactions with POLG and other mitochondrial factors, and elucidate the mechanisms underlying its regulatory role. Furthermore, characterizing different variants of POLG2 can aid in understanding the pathogenic mechanisms associated with mutations, thereby advancing diagnostics and potential gene therapy approaches. Overall, studies on POLG2 recombinant protein not only enhance our knowledge of mitochondrial biology but also hold promise for improving the management of mitochondrial disorders.
