Analytical Data
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Gene name
YTHDF3
- Application
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Alternative Names
FLJ31657; YTH domain family 3; YTH domain family member 3; YTH domain family Protein 3; YTH domain-containing family Protein 3; YTHD3_HUMAN; YTHDF 3; YTHDF3
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z739
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Expression Region
1-585 aa
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AA Sequence
MSATSVDQRPKGQGNKVSVQNGSIHQKDAVNDDDFEPYLSSQTNQSNSYPPMSDPYMPSYYAPSIGFPYSLGEAAWSTAGDQPMPYLTTYGQMSNGEHHYIPDGVFSQPGALGNTPPFLGQHGFNFFPGNADFSTWGTSGSQGQSTQSSAYSSSYGYPPSSLGRAITDGQAGFGNDTLSKVPGISSIEQGMTGLKIGGDLTAAVTKTVGTALSSSGMTSIATNSVPPVSSAAPKPTSWAAIARKPAKPQPKLKPKGNVGIGGSAVPPPPIKHNMNIGTWDEKGSVVKAPPTQPVLPPQTIIQQPQPLIQPPPLVQSQLPQQQPQPPQPQQQQGPQPQAQPHQVQPQQQQLQNRWVAPRNRGAGFNQNNGAGSENFGLGVVPVSASPSSVEVHPVLEKLKAINNYNPKDFDWNLKNGRVFIIKSYSEDDIHRSIKYSIWCSTEHGNKRLDAAYRSLNGKGPLYLLFSVNGSGHFCGVAEMKSVVDYNAYAGVWSQDKWKGKFEVKWIFVKDVPNNQLRHIRLENNDNKPVTNSRDTQEVPLEKAKQVLKIIATFKHTTSIFDDFAHYEKRQEEEEAMRRERNRNKQ
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Molecular Weight
90.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
YTHDF3 is a member of the YTH domain-containing family of proteins, which play critical roles in the regulation of mRNA metabolism, including splicing, stability, translation, and degradation. The YTHDF3 protein binds to N6-methyladenosine (m6A) modified RNA, a prevalent epitranscriptomic modification that significantly influences gene expression and cellular processes. Recent studies have highlighted the importance of YTHDF3 in various biological functions, including cell differentiation, proliferation, and response to stress, as well as its potential implications in diseases such as cancer and neurological disorders. By promoting mRNA translation, YTHDF3 contributes to the post-transcriptional regulatory network, thereby modulating the expression of critical genes involved in cellular signaling pathways. Given its pivotal role in mRNA regulation, YTHDF3 has emerged as a potential therapeutic target, sparking interest in the development of recombinant proteins for further functional characterization. Research on YTHDF3 recombinant protein is essential for elucidating its mechanisms of action, interactions with other cellular components, and the specific mRNA targets it regulates. Understanding these aspects may offer insights into the broader epitranscriptomic landscape and provide new avenues for therapeutic intervention in diseases marked by dysregulation of mRNA metabolism. As such, the production and study of YTHDF3 recombinant protein are critical for advancing our understanding of RNA biology and its implications in health and disease.
