Analytical Data
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Gene name
EIF2B4
- Application
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Alternative Names
EI2BD_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor subunit delta; eIF-2B GDP-GTP exchange factor subunit delta; Eif2b4; EIF2Bdelta; eukaryotic translation initiation factor 2B subunit 4; Translation initiation factor eIF-2B subunit delta
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UI10
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Expression Region
2-523aa
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AA Sequence
AAVAVAVRE DSGSGMKAEL PPGPGAVGRE MTKEEKLQLR KEKKQQKKKR KEEKGAEPET GSAVSAAQCQ VGPTRELPES GIQLGTPREK VPAGRSKAEL RAERRAKQEA ERALKQARKG EQGGPPPKAS PSTAGETPSG VKRLPEYPQV DDLLLRRLVK KPERQQVPTR KDYGSKVSLF SHLPQYSRQN SLTQFMSIPS SVIHPAMVRL GLQYSQGLVS GSNARCIALL RALQQVIQDY TTPPNEELSR DLVNKLKPYM SFLTQCRPLS ASMHNAIKFL NKEITSVGSS KREEEAKSEL RAAIDRYVQE KIVLAAQAIS RFAYQKISNG DVILVYGCSS LVSRILQEAW TEGRRFRVVV VDSRPWLEGR HTLRSLVHAG VPASYLLIPA ASYVLPEVSK VLLGAHALLA NGSVMSRVGT AQLALVARAH NVPVLVCCET YKFCERVQTD AFVSNELDDP DDLQCKRGEH VALANWQNHA SLRLLNLVYD VTPPELVDLV ITELGMIPCS SVPVVLRVKS SDQ
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Molecular Weight
57.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Eukaryotic translation initiation factor 2B (eIF2B) is a critical multi-subunit protein complex that regulates protein synthesis by facilitating the exchange of GDP for GTP on the eIF2 protein, a pivotal step in the initiation of translation. Among its subunits, eIF2B4 plays a vital role in this process, and mutations in the EIF2B4 gene have been implicated in various neurological disorders, particularly in eIF2B-related leukodystrophies. These genetic disorders, characterized by the disruption of myelin formation, manifest in childhood or early adulthood, leading to severe neurological deficits. Research on recombinant EIF2B4 aims to elucidate its structure and function, providing insights into the molecular mechanisms underlying translation initiation and the pathogenesis of related diseases. Studying the recombinant protein allows for the exploration of the biochemical properties and interactions of eIF2B4 within the eIF2B complex, as well as its role in cellular stress response mechanisms. Understanding these molecular dynamics not only enhances our knowledge of fundamental cellular processes but also paves the way for developing targeted therapies for disorders linked to EIF2B4 mutations. Through advances in recombinant protein technology, researchers can produce and purify eIF2B4 for in-depth functional assays, structural analysis, and potential therapeutic applications, ultimately contributing to improved diagnosis and treatment strategies for patients affected by eIF2B-related conditions.
