Analytical Data
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基因名
ENPP4
- Application
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别名
ENPP4. KIAA0879. NPP4
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y6X5
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表达区间
1-453aa
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氨基酸序列
MKLLVILLFSGLITGFRSDSSSSLPPKLLLVSFDGFRADYLKNYEFPHLQNFIKEGVLVEHVKNVFITKTFPNHYSIVTGLYEESHGIVANSMYDAVTKKHFSDSNDKDPFWWNEAVPIWVTNQLQENRSSAAAMWPGTDVPIHDTISSYFMNYNSSVSFEERLNNITMWLNNSNPPVTFATLYWEEPDASGHKYGPEDKENMSRVLKKIDDLIGDLVQRLKMLGLWENLNVIITSDHGMTQCSQDRLINLDSCIDHSYYTLIDLSPVAAILPKINRTEVYNKLKNCSPHMNVYLKEDIPNRFYYQHNDRIQPIILVADEGWTIVLNESSQKLGDHGYDNSLPSMHPFLAAHGPAFHKGYKHSTINIVDIYPMMCHILGLKPHPNNGTFGHTKCLLVDQWCINLPEAIAIVIGSLLVLTMLTCLIIIMQNRLSVPRPFSRLQLQEDDDDPLIG
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分子量
78 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ENPP4 (Ectonucleotide Triphosphate Diphosphohydrolase 4) is an enzyme belonging to the ectoenzyme family that plays a critical role in regulating nucleotide metabolism, particularly in the hydrolysis of extracellular ATP and other nucleotides. Recent research has indicated that ENPP4 is involved in various biological processes, including cellular signaling, inflammation, and immune responses. Its dysregulation has been linked to pathological conditions such as cancer and metabolic disorders. Given its potential as a therapeutic target, the production of recombinant ENPP4 protein has gained significant interest. Researchers aim to characterize its enzymatic activity and understand the structural basis of its function to develop inhibitors or modulators that could serve as novel treatments. Additionally, studying the recombinant protein allows for deeper insights into its interactions with other molecules, contributing to the broader understanding of nucleotide metabolism and its implications in health and disease. The advancements in recombinant DNA technology have facilitated the efficient production of ENPP4, paving the way for innovative approaches in biomedical research.
