Analytical Data
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基因名
FBXL5
- Application
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别名
F box and leucine rich repeat protein 5; F box protein FBL5; F box/LRR repeat protein 5; F-box and leucine-rich repeat protein 5; F-box protein FBL4/FBL5; F-box/LRR-repeat protein 5; FBL4
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UKA1
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表达区间
1-691aa
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氨基酸序列
MAPFPEEVDVFTAPHWRMKQLVGLYCDKLSKTNFSNNNDFRALLQSLYATFTEFKMHEQIENEYIIGLLQQRSQTIYNVHSDNKLSEMLSLFEKGLKNVKNEYEQLNYAKQLKERLEAFTRDFLPHMKEEEEVFQPMLMEYFTYEELKDIKKKVIAQHCSQKDTAELLRGLSLWNHAEERQKFFKYSVDEKSDKEAEVSEHSTGITHLPPEVMLSIFSYLNPQELCRCSQVSMKWSQLTKTRSLWKHLYPVHWARGDWYSGPATELDTEPDDEWVKNRKDESRAFHEWDEDADIDESEESAEESIAISIAQMEKRLLHGLIHNVLPYVGTSVKTLVLAYSSAVSSKMVRQILELCPNLEHLDLTQTDISDSAFDSWSWLGCCQSLRHLDLSGCEKITDVALEKISRALGILTSHQSGFLKTSTSKITSTAWKNKDITMQSTKQYACLHDLTNKGIGEEIDNEHPWTKPVSSENFTSPYVWMLDAEDLADIEDTVEWRHRNVESLCVMETASNFSCSTSGCFSKDIVGLRTSVCWQQHCASPAFAYCGHSFCCTGTALRTMSSLPESSAMCRKAARTRLPRGKDLIYFGSEKSDQETGRVLLFLSLSGCYQITDHGLRVLTLGGGLPYLEHLNLSGCLTITGAGLQDLVSACPSLNDEYFYYCDNINGPHADTASGCQNLQCGFRACCRSGE
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分子量
105 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FBXL5, a member of the F-box protein family, plays a crucial role in the regulation of various cellular processes, primarily through its involvement in ubiquitin-proteasome pathways. Its function is vital for regulating the stability of important substrates, including hypoxia-inducible factors (HIFs), which are key players in cellular responses to oxygen availability. Recent studies have indicated that FBXL5 acts as a central regulator in the degradation of HIF-1α, thus influencing processes such as angiogenesis and cellular metabolism in response to hypoxic conditions. Given its pivotal functions, FBXL5 has garnered significant attention in the context of cancer research, where altered expression levels may contribute to tumorigenicity and metastasis. Moreover, FBXL5's interaction with iron metabolism has been linked to the regulation of iron-responsive element-binding proteins, further highlighting its multifaceted role in cellular homeostasis. In this light, the production and characterization of recombinant FBXL5 protein are essential for elucidating its molecular mechanisms, understanding its role in various diseases, and exploring its potential as a therapeutic target. By leveraging recombinant DNA technology, researchers aim to generate purified FBXL5 for biochemical assays, structural studies, and functional analyses that could ultimately lead to novel insights into its contributions to disease pathogenesis and offer new avenues for treatment strategies.
