Analytical Data
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Gene name
TTF2
- Application
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Alternative Names
F2; Factor 2; Factor2; HuF 2; HuF2; Human factor 2; Lodestar homolog; Lodestar Protein; RNA polymerase II termination factor; Transcription release factor 2; Transcription termination factor 2; Transcription termination factor RNA polymerase II; TTF2; TTF2_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UNY4
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Expression Region
1-373 aa
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AA Sequence
MTAESGPPPPQPEVLATVKEERGETAAGAGVPGEATGRGAGGRRRKRPLQRGKPPYSYIALIAMAIAHAPERRLTLGGIYKFITERFPFYRDNPKKWQNSIRHNLTLNDCFLKIPREAGRPGKGNYWALDPNAEDMFESGSFLRRRKRFKRSDLSTYPAYMHDAAAAAAAAAAAAAAAAIFPGAVPAARPPYPGAVYAGYAPPSLAAPPPVYYPAASPGPCRVFGLVPERPLSPELGPAPSGPGGSCAFASAGAPATTTGYQPAGCTGARPANPSAYAAAYAGPDGAYPQGAGSAIFAAAGRLAGPASPPAGGSSGGVETTVDFYGRTSPGQFGALGACYNPGGQLGGASAGAYHARHAAAYPGGIDRFVSAM
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Molecular Weight
67.43 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TTF2 (Transcription Termination Factor 2) is a crucial protein involved in the regulation of gene expression by facilitating the termination of transcription processes in eukaryotic cells. Its role extends beyond the mere termination of transcription; TTF2 contributes to the accuracy of RNA polymerase II in completing the transcription cycle, thereby preventing premature termination and ensuring the synthesis of full-length messenger RNA (mRNA). The study of TTF2 recombinant proteins has garnered increasing interest due to its potential implications in understanding various cellular mechanisms, including gene regulation, cellular response to stress, and the maintenance of genome integrity. Additionally, TTF2 has been implicated in certain pathologies, such as cancer, where dysregulation of transcriptional processes can lead to uncontrolled cell growth. By exploring the structure and function of TTF2 through recombinant protein technology, researchers aim to elucidate its biochemical properties, interactions with other molecules, and the pathways influenced by its activity. This research not only enhances our fundamental understanding of transcriptional regulation but also opens avenues for therapeutic interventions targeting transcriptional dysregulation in diseases. The ability to produce TTF2 in a recombinant system enables detailed biochemical assays, structural studies, and the development of potential inhibitors, making it a significant focus in molecular biology and biochemistry.
