Analytical Data
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Gene name
FBXO33
- Application
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Alternative Names
F box only protein 33; F-box only protein 33; Fbx33; FBX33_HUMAN; FBXO33
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z6M2
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Expression Region
1-555aa
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AA Sequence
MLLFLSVPQPRPPGARTRAGAARVARWRRLRLQQLRRLRGLLRVLRGRPGAGSRRRGRMALCGQAAGAASLPSELIVHIFSFLPAPDRLRASASCSHWRECLFYPALWPQLRICLRVSPAEQPRLEFLMRKCGWFVRELRVEFAAENYLSGGGPGDGGGADTGTGGEEVEALQLSARWLEVLRTYLELVLCVLVSIRNNRNLQKFSLFGDISVLQQQGSLSNTYLSKVDPDGKKIKQIQQLFEEILSNSRQLKWLSCGFMLEIVTPTSLSSLSNAVANTMEHLSLLDNNIPGNSTLITAVELERFVNLHSLALDFCDFTAEMARVLTDSNHVPLQRLSLLVHNVSVMHKSLDNMPNDEHWKALSRKSTSFRVYIMAFDIKSEDMLKILKPSIPLERIHFDSYITCVSGAIVDLISRQYDKFLTHFILMNDVIDTSGFPDLSDNRNEDPLVLLAWRCTKLSLLAIHGYTVWAHNLIAIARLRGSDLKVLEVTEESIDFDQGELADQDVDPVHNLIEQVSLGLGQPWHAVMDIESLSVFTEPNRHFYREMQSFSEDI
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Molecular Weight
89 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FBXO33, a member of the F-box protein family, plays a pivotal role in cellular processes by functioning as a substrate recognition component of SCF (SKP1-CUL1-F-box) ubiquitin ligases. These complexes are crucial for regulating protein degradation through the ubiquitin-proteasome pathway, which is essential for maintaining cellular homeostasis, influencing cell cycle progression, and modulating various signaling pathways. Dysregulation of FBXO33 has been implicated in a range of diseases, including cancer, where altered ubiquitin-mediated degradation can lead to the accumulation of oncogenic proteins or loss of tumor suppressor functions. Recent studies have highlighted the potential of FBXO33 as a therapeutic target, as its manipulation could restore proper protein turnover and alleviate disease phenotypes. Furthermore, understanding the structure-function relationship of FBXO33 through recombinant protein studies can provide insights into its precise mechanisms of action and interactions with substrates. This knowledge is vital for developing targeted therapies that exploit the vulnerabilities in the ubiquitin system that diseases present. Given the complexity and significance of FBXO33 in cellular regulation, ongoing research aims to elucidate its role in various biological contexts and its potential as a biomarker or therapeutic target in diseases characterized by proteostasis disruption.
