Analytical Data
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基因名
USP4
- Application
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别名
Deubiquitinating enzyme 4; MGC149848; MGC149849; Proto oncogene; Protooncogene; Ubiquitin carboxyl terminal hydrolase 4; Ubiquitin carboxyl-terminal hydrolase 4; Ubiquitin specific peptidase 4 (proto oncogene); Ubiquitin specific peptidase 4 (protooncogene); Ubiquitin specific peptidase 4; Ubiquitin specific processing protease 4; Ubiquitin specific protease 4 (proto oncogene); Ubiquitin specific protease 4 (protooncogene); Ubiquitin specific protease 4; Ubiquitin specific protease proto oncogene; Ubiquitin specific protease protooncogene; Ubiquitin thioesterase 4; Ubiquitin thiolesterase 4; Ubiquitin-specific-processing protease 4; Ubiquitous nuclear Protein homolog; UBP4_HUMAN; UNP; UNPH; USP 4; USP4
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13107
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表达区间
1-963 aa
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氨基酸序列
MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKSSTAPSRNFTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVSRGGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEFYKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSMDTN
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分子量
135 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
USP4 (Ubiquitin-Specific Protease 4) is a deubiquitinating enzyme that plays a critical role in regulating various cellular processes, including protein stability, signal transduction, and gene expression. Dysregulation of USP4 has been implicated in several pathologies, including cancer, neurodegenerative diseases, and immune disorders, making it an attractive target for therapeutic intervention. Studies suggest that USP4 may modulate the activity of key signaling pathways, such as the NF-κB and Wnt/β-catenin pathways, by removing ubiquitin chains from target proteins, thus influencing their degradation and function. The expression and activity of USP4 can be altered in disease states, contributing to tumorigenesis and resistance to therapy. Research into USP4 recombinant protein has provided insights into its structure-function relationships, enzymatic mechanisms, and interactions with substrates. Understanding the molecular basis of USP4’s activity is not only crucial for elucidating its role in normal cellular function but also for developing novel therapeutics that can selectively target USP4 to restore normal signaling pathways in diseases where ubiquitination signaling is disrupted. Hence, the exploration of USP4 recombinant protein is pivotal for advancing our comprehension of its biological significance and potential as a drug target in various diseases.
