Analytical Data
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基因名
RNF55
- Application
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别名
RNF55；MURF2；RNF29；Tripartite motif-containing Protein 55
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P22681
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表达区间
1-906aa
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氨基酸序列
MAGNVKKSSGAGGGSGSGGSGSGGLIGLMKDAFQPHHHHHHHLSPHPPGT VDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILS RYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRN LTKLSLIFSHMLAELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKTIV PWKSFRQALHEVHPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQP WSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRL GQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLT GLCEPTPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLM CTSCLTSWQESEGQGCPFCRCEIKGTEPIVVDPFDPRGSGSLLRQGAEGA PSPNYDDDDDERADDTLFMMKELAGAKVERPPSPFSMAPQASLPPVPPRL DLLPQRVCVPSSASALGTASKAASGSLHKDKPLPVPPTLRDLPPPPPPDR PYSVGAESRPQRRPLPCTPGDCPSRDKLPPVPSSRLGDSWLPRPIPKVPV SAPSSSDPWTGRELTNRHSLPFSLPSQMEPRPDVPRLGSTFSLDTSMSMN SSPLVGPECDHPKIKPSSSANAIYSLAARPLPVPKLPPGEQCEGEEDTEY MTPSSRPLRPLDTSQSSRACDCDQQIDSCTYEAMYNIQSQAPSITESSTF GEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSF GWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASA ATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISS PAHVAT
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分子量
126 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF55, a member of the RING finger protein family, has garnered attention in recent years for its potential role in various cellular processes, including protein ubiquitination and regulation of signal transduction pathways. Ubiquitination is a critical post-translational modification that influences protein stability, localization, and activity, making RNF55 a key player in maintaining cellular homeostasis. Studies suggest that RNF55 is involved in immune responses and cellular stress management, highlighting its significance in diseases such as cancer and autoimmune disorders. Researchers are increasingly focused on understanding the exact mechanisms by which RNF55 operates at the molecular level, particularly its interactions with other proteins and its impact on gene expression. The recombinant expression of RNF55 in various model systems enables detailed functional analyses, paving the way for potential therapeutic strategies that target its ubiquitin ligase activity. Exploring the structure-function relationship of RNF55 through recombinant proteins can provide insights into its regulatory roles and identify novel approaches for modulating its activity in disease contexts. Thus, the study of RNF55 and its recombinant protein forms represents a promising avenue for further elucidating the complexities of cellular regulation and developing targeted interventions in related pathologies.
