Analytical Data
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基因名
AP
- Application
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别名
AP；AP2TF；TFAP2；Transcription factor AP-2-alpha
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H4A4
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表达区间
1-650aa
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氨基酸序列
MASGEHSPGSGAARRPLHSAQAVDVASASNFRAFELLHLHLDLRAEFGPP GPGAGSRGLSGTAVLDLRCLEPEGAAELRLDSHPCLEVTAAALRRERPGS EEPPAEPVSFYTQPFSHYGQALCVSFPQPCRAAERLQVLLTYRVGEGPGV CWLAPEQTAGKKKPFVYTQGQAVLNRAFFPCFDTPAVKYKYSALIEVPDG FTAVMSASTWEKRGPNKFFFQMCQPIPSYLIALAIGDLVSAEVGPRSRVW AEPCLIDAAKEEYNGVIEEFLATGEKLFGPYVWGRYDLLFMPPSFPFGGM ENPCLTFVTPCLLAGDRSLADVIIHEISHSWFGNLVTNANWGEFWLNEGF TMYAQRRISTILFGAAYTCLEAATGRALLRQHMDITGEENPLNKLRVKIE PGVDPDDTYNETPYEKGFCFVSYLAHLVGDQDQFDSFLKAYVHEFKFRSI LADDFLDFYLEYFPELKKKRVDIIPGFEFDRWLNTPGWPPYLPDLSPGDS LMKPAEELAQLWAAEELDMKAIEAVAISPWKTYQLVYFLDKILQKSPLPP GNVKKLGDTYPSISNARNAELRLRWGQIVLKNDHQEDFWKVKEFLHNQGK QKYTLPLYHAMMGGSEVAQTLAKETFASTASQLHSNVVNYVQQIVAPKGS
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分子量
99 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The research on AP (Alkaline Phosphatase) recombinant proteins has gained significant attention due to their diverse applications in biochemistry and molecular biology. Alkaline phosphatase is an essential enzyme that catalyzes the hydrolysis of phosphate groups from various molecules, playing a critical role in metabolic processes. The ability to produce recombinant forms of this enzyme allows for greater control over its properties and enhances its functionality for specific applications. Researchers have focused on the optimization of expression systems, such as bacterial, yeast, and mammalian cells, to achieve high yields and proper folding of AP proteins. These recombinant enzymes are increasingly used in various fields, including diagnostic assays, biopharmaceutical production, and molecular biology techniques, like dephosphorylation of nucleic acids prior to cloning. Moreover, the study of AP in a recombinant form has also facilitated advancements in understanding enzyme kinetics and structural biology, contributing to broader scientific knowledge. Overall, the ongoing research into AP recombinant proteins not only enhances the practical utility of this enzyme but also broadens our understanding of enzyme function and regulation within biological systems.
