Analytical Data
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Gene name
G6PC3
- Application
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Alternative Names
G6PC3; UGRPGlucose-6-phosphatase 3; G-6-Pase 3; G6Pase 3; EC 3.1.3.9; Glucose-6-phosphatase beta; G6Pase-beta; Ubiquitous glucose-6-phosphatase catalytic subunit-related protein
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BUM1
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Expression Region
1-346aa
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AA Sequence
MESTLGAGIVIAEALQNQLAWLENVWLWITFLGDPKILFLFYFPAAYYASRRVGIAVLWISLITEWLNLIFKWFLFGDRPFWWVHESGYYSQAPAQVHQFPSSCETGPGSPSGHCMITGAALWPIMTALSSQVATRARSRWVRVMPSLAYCTFLLAVGLSRIFILAHFPHQVLAGLITGAVLGWLMTPRVPMERELSFYGLTALALMLGTSLIYWTLFTLGLDLSWSISLAFKWCERPEWIHVDSRPFASLSRDSGAALGLGIALHSPCYAQVRRAQLGNGQKIACLVLAMGLLGPLDWLGHPPQISLFYIFNFLKYTLWPCLVLALVPWAVHMFSAQEAPPIHSS
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Molecular Weight
65.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
G6PC3 (Glucose-6-phosphatase catalytic subunit 3) is an essential enzyme in glucose metabolism, primarily involved in gluconeogenesis and glycogenolysis. Mutations in the G6PC3 gene lead to a rare autosomal recessive disorder known as G6PC3 deficiency, characterized by severe congenital neutropenia, increased susceptibility to infections, and metabolic complications. The enzyme's role in regulating blood glucose levels highlights its significance in maintaining energy homeostasis. Research on the recombinant G6PC3 protein has gained traction as it provides insights into the enzyme's structure-function relationships and the underlying mechanisms of its associated disorders. The production of recombinant G6PC3 allows for detailed biochemical analyses and the exploration of potential therapeutic strategies, including enzyme replacement therapy. Furthermore, understanding the regulatory pathways involving G6PC3 can lead to advancements in treatments for related metabolic disorders, thereby improving patient outcomes. Given the enzyme's critical role in cellular metabolism, ongoing studies aim to elucidate its full biological significance and potential implications in broader metabolic pathways. This research not only enhances our understanding of G6PC3 but also contributes to the field of metabolic disease research and therapeutic development.
