Analytical Data
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Gene name
GFI1
- Application
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Alternative Names
GFI 1; GFI1; GFI1_HUMAN; Growth factor independence 1; Growth factor independent 1; Growth factor independent protein 1; SCN2; Zinc finger protein 163; Zinc finger protein Gfi 1; Zinc finger protein Gfi-1; Zinc finger protein Gfi1; ZNF 163; ZNF163
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99684
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Expression Region
1-422aa
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AA Sequence
MPRSFLVKSK KAHSYHQPRS PGPDYSLRLE NVPAPSRADS TSNAGGAKAE PRDRLSPESQ LTEAPDRASA SPDSCEGSVC ERSSEFEDFW RPPSPSASPA SEKSMCPSLD EAQPFPLPFK PYSWSGLAGS DLRHLVQSYR PCGALERGAG LGLFCEPAPE PGHPAALYGP KRAAGGAGAG APGSCSAGAG ATAGPGLGLY GDFGSAAAGL YERPTAAAGL LYPERGHGLH ADKGAGVKVE SELLCTRLLL GGGSYKCIKC SKVFSTPHGL EVHVRRSHSG TRPFACEMCG KTFGHAVSLE QHKAVHSQER SFDCKICGKS FKRSSTLSTH LLIHSDTRPY PCQYCGKRFH QKSDMKKHTF IHTGEKPHKC QVCGKAFSQS SNLITHSRKH TGFKPFGCDL CGKGFQRKVD LRRHRETQHG LK
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Molecular Weight
45.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GFI1 (Growth Factor Independence 1) is a key transcription factor that plays a crucial role in hematopoiesis, neuronal development, and cell differentiation. Its function is primarily associated with the regulation of gene expression during these processes, making it a significant protein in both normal physiological and pathological states. Research has shown that GFI1 is involved in various cancers, particularly acute myeloid leukemia (AML), where its overexpression can contribute to tumorigenesis and the maintenance of leukemia stem cells. Understanding the structure and function of GFI1 through the study of its recombinant protein is essential for uncovering its mechanisms of action. This research could pave the way for potential therapeutic strategies targeting GFI1 in cancers or other diseases characterized by dysregulated hematopoiesis and development. By producing recombinant GFI1 proteins, scientists can explore its interactions with other cellular components, its regulatory networks, and the implications of post-translational modifications in its activity. Additionally, recombinant GFI1 can be a valuable tool for drug screening and development, as modulating its activity may yield innovative approaches to treat related conditions. Overall, the study of GFI1 recombinant protein not only enhances our understanding of its biological significance but also opens avenues for novel therapeutic interventions in hematological malignancies and neurodevelopmental disorders.
