Analytical Data
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Gene name
AMCase/CHIA
- Application
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Alternative Names
/
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Species
Zea mays
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
D0EM57
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Expression Region
27-278aa
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Molecular Weight
30.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AMCase (acidic mammalian chitinase) and CHIA (chitinase 3-like 1) are significant proteins belonging to the glycoside hydrolase family, which play vital roles in chitin degradation and immune responses. AMCase was first identified in mammalian systems where it is predominantly expressed in the lungs and is implicated in various biological processes, including allergy and asthma. Its ability to break down chitin, a structural polysaccharide found in the exoskeletons of insects and the cell walls of fungi, positions it as a potential therapeutic target for diseases associated with chitin-containing pathogens. CHIA, on the other hand, is a member of the chitinase-like protein family and is involved in modulating inflammatory responses and tissue repair. Research has shown that CHIA might be linked to obesity and metabolic disorders. The recombinant expression of AMCase and CHIA allows for in-depth studies of their structure-function relationships and biological activities. This provides essential insights into their roles in health and disease, as well as potential applications in biotechnology and pharmacology. The successful production of these recombinant proteins can facilitate the development of novel therapeutics that harness their chitinase activities, promoting further exploration of their therapeutic potential in treating allergic diseases, infections, and metabolic disorders.
