Analytical Data
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Gene name
TIM
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简介
TIM protein is a highly efficient metabolic enzyme that catalyzes the rapid interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) during glycolysis and gluconeogenesis. Its key role in glucose metabolism is complemented by an additional responsibility: the production of methylglyoxal, a reactive cytotoxic by-product. TIM Protein, Human (His) is the recombinant human-derived TIM protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
Triosephosphate Isomerase; TIM; Triose-Phosphate Isomerase; TPI1; TPI
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P60174
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Expression Region
M1-Q249
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AA Sequence
MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ
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Protein Length
Full Length
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Molecular Weight
30.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TIM (Triosephosphate Isomerase) is a crucial enzyme in the glycolytic pathway, catalyzing the interconversion of dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. This enzyme plays a significant role in energy metabolism and is highly conserved across various organisms, highlighting its essential function in cellular processes. Research on TIM has gained prominence due to its implications in various diseases, including cancer and neurodegenerative disorders, where TIM's dysregulation is often observed. Moreover, TIM's structural features, particularly its highly conserved active site, have made it a subject of interest in enzyme engineering and biocatalysis. Recent advancements in protein engineering techniques, such as directed evolution and crystallography, have allowed scientists to better understand the enzyme's structure-function relationships, paving the way for potential therapeutic applications and biotechnological innovations. The study of TIM and its recombinant forms also offers insights into evolutionary biology, as variations in TIM across species can reveal adaptive mechanisms to different environmental conditions. Overall, TIM remains a key focus in biochemistry and molecular biology, linking fundamental metabolic processes to broader biological insights and applications.
