Analytical Data
-
基因名
Syndecan-3
-
简介
Syndecan-3 Protein, Human (HEK293, His) is a Dendritic cells (DCs)-specific heparan sulfate (HS) proteoglycan that captures HIV-1 through interaction with the HIV-1 envelope glycoprotein gp120. SDC3 is involved in the regulation of growth factor signalling, Notch and BMP signalling, adhesion and migration, proliferation and differentiation. Syndecan-3 is associated with the control of feeding behaviour and the generation of cerebellar fibrillar plaques in Alzheimer鈥檚 disease[1][2][3]. Syndecan-3 Protein, Human (HEK293, His) is the recombinant human-derived Syndecan-3 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
-
生物活性
Measured by the ability of the immobilized protein to inhibit the adhesion of Saos‑2 human osteosarcoma cells to human Fibronectin. The ED50 for this effect is 0.5-2.0 μg/ml in the presence of 5 μg/mL Fibronectin. Measured by the ability of the immobilized protein to inhibit the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. The ED50 for this effect is 1.505 μg/mL in the presence of 5 μg/mL Fibronectin, corresponding to a specific activity is 664.452 units/mg.
-
别名
N-Syndecan; SDC3; SDCN; Syndecan-3; SYND3syndecan proteoglycan 3
-
种属
Human
-
表达系统
HEK293
-
标签
C-6*His
-
纯度
Greater than 95% as determined by SDS-PAGE.
-
蛋白编号
ABM82826
-
表达区间
M1-K311
-
氨基酸序列
MAIAYLGSSCPSQPPSSLALSLSPTPSDFEQESGIETAMRFSPDVALAVSTTPFEELPSERPTLEPATSPLVVTEVPEEPSQRATTVSTTMATTAATSTGDPTVATVPATVATATPSTPAAPPFTATTAVIRTTGVRRLLPLPLTTVATARATTPEAPSPPTTAAVLDTEAPTPRLVSTATSRPRALPRPATTQEPDIPERSTLPLGTTAPGPTEVAQTPTPETFLTTIRDEPEVPVSGGPSGDFELPEEETTQPDTANEVVAVGGAAAKASSPPGTLPKGARPGPGLLDNAIDSGSSAAQLPQKSILERK
-
蛋白长度
Partial
-
分子量
58-100 kDa band in SDS-PAGE under reducing conditions.
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Syndecan-3, a member of the syndecan family of transmembrane heparan sulfate proteoglycans, plays a significant role in cellular processes such as adhesion, proliferation, and migration, which are crucial for tissue development and repair. This protein functions primarily as a co-receptor for various growth factors and extracellular matrix molecules, facilitating intercellular communication and signaling pathways. Recent studies have highlighted its involvement in neurodevelopment and its potential role in the pathophysiology of neurodegenerative diseases. The production of recombinant Syndecan-3 protein has become a valuable tool for researchers aiming to elucidate its specific functions and mechanisms of action. By using recombinant DNA technology, scientists can generate large quantities of this protein in a controlled manner, enabling detailed biochemical assays and structural studies. Additionally, understanding Syndecan-3's interactions with ligands and its signaling mechanisms may lead to novel therapeutic approaches for conditions associated with its dysregulation. Thus, ongoing research into recombinant Syndecan-3 not only enhances our comprehension of cellular biology but also opens the door for innovative strategies in drug development and regenerative medicine.
