Analytical Data
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Gene name
CDK1-CCNB1
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简介
The CDK1/CDC2-cyclin-B complex is a key regulator in the cell cycle. It plays an important role in cell division and development and regulates a variety of cellular activities, including chromosome segregation, nuclear membrane rupture, cytokinesis, etc. The activity of CDK1 is regulated by a variety of phosphorylation and dephosphorylation, thereby controlling the progression of the cell cycle and the DNA repair process. CDK1-CCNB1 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK1-CCNB1, expressed by Sf9 insect cells, with N-GST, N-Flag, N-His labeled tag.
- Application
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Species
Human
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Source
Baculovirus
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Tag
N-GST;N-Flag;N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06493-1 (M1-M297)&P14635-1
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Expression Region
P06493-1 (M1-M297)&P14635-1 (N165-V433)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDK1 (Cyclin-dependent kinase 1) is a crucial regulator of the cell cycle, particularly in the transition from the G2 phase to mitosis. It functions in association with its regulatory partner, Cyclin B1 (CCNB1), forming a complex that is essential for the initiation of mitotic events. The CDK1-CCNB1 complex phosphorylates various substrates, resulting in chromatin condensation, spindle assembly, and ultimately, cell division. Dysregulation of CDK1 activity has been implicated in various cancers, where aberrant cell division contributes to tumorigenesis. Research into the CDK1-CCNB1 complex is important not only for understanding fundamental cell cycle mechanisms but also for identifying potential therapeutic targets in cancer treatment. Recombinant proteins of CDK1 and CCNB1 facilitate the study of their biochemical properties and interactions, allowing researchers to investigate how alterations in this complex might affect mitotic progression and contribute to disease. By analyzing the structure-function relationships of CDK1 and CCNB1, researchers aim to uncover new insights into cell cycle regulation and its implications in cancer biology. Additionally, the development of small molecules targeting the CDK1-CCNB1 complex may lead to novel therapeutic strategies for cancer patients, making the study of this recombinant protein complex highly relevant in both basic and applied biomedical research.
