Analytical Data
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Gene name
NMNAT1
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简介
The NMNAT1 protein catalyzes the formation of NAD(+) from NMN and ATP and efficiently uses deamidated NaMN and triazofurane monophosphate as substrates. NMNAT1 Protein, Human (sf9, His) is the recombinant human-derived NMNAT1 protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Application
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Alternative Names
Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1; NMNAT1; NMNAT
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Species
Human
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Source
Baculovirus
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HAN9
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Expression Region
M1-T279
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Protein Length
Full Length
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Molecular Weight
34 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NMNAT1 (Nicotinamide Mononucleotide Adenylyltransferase 1) is an essential enzyme in the NAD+ biosynthesis pathway, playing a pivotal role in cellular energy metabolism and maintaining cellular homeostasis. Its significance extends beyond basic cellular functions; NMNAT1 is implicated in neuroprotection, as it is predominantly expressed in the brain. Research has demonstrated that NMNAT1 protects neurons from oxidative stress and apoptosis, indicating its potential as a therapeutic target in neurodegenerative diseases like Alzheimer’s and Parkinson’s. The reconstitution of NMNAT1 as a recombinant protein allows for detailed biochemical studies, providing insight into its catalytic mechanisms, substrate specificity, and regulation. Moreover, studying NMNAT1's structure and function can elucidate its role in NAD+ metabolism and its broader implications in aging and related disorders. By exploring NMNAT1 through recombinant protein technology, researchers aim to uncover novel therapeutic strategies to modulate its activity and enhance NAD+ levels in cells, potentially offering new avenues for treatment in conditions associated with energy metabolism dysregulation. This research emphasizes the importance of NMNAT1 not only in understanding fundamental biochemical pathways but also in developing interventions for age-related and neurodegenerative diseases.
