Analytical Data
-
Gene name
NETO1
-
简介
The NETO1 protein complexly maintains neuronal circuits as an auxiliary subunit of NMDARs, which is critical for the abundance of GRIN2A-containing NMDARs in the postsynaptic density. It regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, particularly in spatial learning and memory. NETO1 Protein, Human (HEK293, His) is the recombinant human-derived NETO1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
-
Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human Amyloid Precursor binds with an ED50 of 0.6861 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human Amyloid Precursor binds with an ED50 of 0.6861 μg/mL.
-
Alternative Names
Neuropilin and tolloid-like protein 1; NETO1; BTCL1
-
Species
Human
-
Source
HEK293
-
Tag
C-6*His
-
Purity
Greater than 95% as determined by SDS-PAGE.
-
Uniprot
Q8TDF5-3
-
Expression Region
T23-T344
-
AA Sequence
TKKGTEKQTTSETQKSVQCGTWTKHAEGGIFTSPNYPSKYPPDRECIYIIEAAPRQCIELYFDEKYSIEPSWECKFDHIEVRDGPFGFSPIIGRFCGQQNPPVIKSSGRFLWIKFFADGELESMGFSARYNFTPDPDFKDLGALKPLPACEFEMGGSEGIVESIQIMKEGKATASEAVDCKWYIRAPPRSKIYLRFLDYEMQNSNECKRNFVAVYDGSSSVEDLKAKFCSTVANDVMLRTGLGVIRMWADEGSRNSRFQMLFTSFQEPPCEGNTFFCHSNMCINNTLVCNGLQNCVYPWDENHCKEKRKTSLLDQLTNTSGT
-
Protein Length
Extracellular Domain
-
Molecular Weight
40-50 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NETO1 (Neurotrophin-Dependent Endosomal Transporter 1) is a protein that plays a significant role in the neurotrophic signaling pathways, particularly in synaptic plasticity and neuronal survival. Research on NETO1 has gained momentum due to its involvement in the transport and signaling of neurotrophins, which are crucial for the development and maintenance of the nervous system. Abnormalities in NETO1 expression or function have been linked to neurodevelopmental disorders and neurodegenerative diseases, making it a potential target for therapeutic interventions. The understanding of NETO1's structure and its molecular interactions can provide insights into its role in neuronal health and disease. Recent studies have employed recombinant protein techniques to investigate the functional properties of NETO1, aiming to elucidate its mechanism of action at the cellular level. This research is particularly relevant for developing strategies to modulate NETO1 activity in conditions where neurotrophin signaling is disrupted. Overall, the investigation of NETO1 and its recombinant protein is pivotal in advancing our knowledge of neuronal function and may contribute to innovative treatments for various neurological disorders.
