Analytical Data
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Gene name
GCLM
- Application
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Alternative Names
GLCLR; GECS; GCL; Gamma Glutamylcysteine Synthetase; Glutamate Cysteine Ligase Regulatory; Gamma-ECS regulatory subunit; Glutamate cysteine ligase modifier subunit
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48507
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Expression Region
Ser40~Ser251
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Molecular Weight
28kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GCLM (glutamate-cysteine ligase modifier subunit) is an essential component of the γ-glutamylcysteine synthetase enzyme complex, which plays a critical role in the synthesis of glutathione, a key antioxidant in cellular defense against oxidative stress. The regulation of glutathione levels is crucial for maintaining cellular homeostasis and protecting cells from damage caused by reactive oxygen species (ROS). Dysregulation of GCLM has been implicated in various pathological conditions, including neurodegenerative diseases, cancer, and metabolic disorders. Understanding the structure and function of GCLM is therefore vital for elucidating its role in disease processes and for developing therapeutic strategies aimed at modulating glutathione levels. Recombinant GCLM proteins have been produced for enzymatic assays, structural studies, and potential therapeutic applications. By investigating the biochemical properties of GCLM and its interaction with other cellular components, researchers aim to uncover novel insights into cellular oxidative stress responses and to identify potential targets for drug development. The ongoing research on GCLM reaffirms its significance in both basic and applied biomedical sciences, making it a focal point for understanding disease mechanisms and exploring innovative treatment approaches.
