Analytical Data
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Gene name
NSD3
- Application
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Alternative Names
Nuclear SET domain-containing protein 3 (Protein whistle) (WHSC1-like 1 isoform 9 with methyltransferase activity to lysine) (Wolf-Hirschhorn syndrome candidate 1-like protein 1) (WHSC1-like protein 1) (WHSC1L1)
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Species
Human
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Source
Yeast
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BZ95
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Expression Region
1-645aa
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Molecular Weight
73.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NSD3 (Nuclear Receptor Binding SET Domain Protein 3) is a member of the NSD (Nuclear SET Domain) family of histone methyltransferases, which play crucial roles in gene expression regulation and chromatin remodeling. It specifically di-methylates histone H3 at lysine 36 (H3K36me2), a mark associated with active transcription and DNA damage repair. Dysregulation of NSD3 has been implicated in various cancers, including leukemia, indicating its potential as a therapeutic target. Research has focused on understanding the structural and functional properties of NSD3, as its aberrant expression can contribute to oncogenic processes. Recombinant NSD3 proteins are essential for studying its enzymatic activity, interaction with other epigenetic modifiers, and its role in cellular pathways. Additionally, the production of NSD3 as a recombinant protein allows for high-throughput screening of small molecules that could inhibit its activity, paving the way for novel cancer treatments. Understanding NSD3's mechanism could unlock new strategies for targeting malignancies associated with its dysregulation. Thus, research on the recombinant NSD3 protein is pivotal for developing insights into its involvement in gene regulation and for identifying potential therapeutic approaches in cancer treatment.
