Analytical Data
-
Gene name
BRF1
- Application
-
Alternative Names
TFIIIB90 (hTFIIIB90) (B-related factor 1) (BRF-1) (hBRF)
-
Species
Human
-
Source
Yeast
-
Tag
N- His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q92994
-
Expression Region
1-677aa
-
Molecular Weight
75.3
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BRF1 (BRAF-regulated factor 1) is a crucial component of the transcriptional machinery, particularly in the context of RNA polymerase II-mediated transcription. It plays a significant role in the recruitment of transcription factors and co-activators to gene promoters. Dysregulation of BRF1 has been linked to various diseases, including cancer, where its overexpression can lead to aberrant gene expression profiles and tumorigenesis. The study of BRF1 recombinant protein has gained traction as researchers aim to elucidate its functional mechanisms and interactions with other cellular proteins. By producing and purifying BRF1 as a recombinant protein, scientists can investigate its structural properties, functional activity, and interaction networks in vitro. Furthermore, understanding the precise role of BRF1 can offer insights into normal cellular processes and the molecular basis of diseases, paving the way for potential therapeutic strategies. The ongoing research into BRF1 emphasizes the importance of post-transcriptional regulation in gene expression and highlights its potential as a biomarker or target for drug development.
