Analytical Data
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Gene name
CDC27
- Application
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Alternative Names
APC3; ANAPC3; CDC27Hs; HNUC; Anaphase Promoting Complex Subunit 3
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P30260
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Expression Region
Asn17~Ser231
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Molecular Weight
29kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CDC27, a crucial component of the anaphase-promoting complex/cyclosome (APC/C), plays a significant role in regulating the cell cycle by mediating the ubiquitination and subsequent degradation of cell cycle proteins. Research into CDC27 has gained prominence due to its involvement in various cellular processes, including the transition from metaphase to anaphase and the maintenance of genomic stability. Mutations or dysregulation of CDC27 have been implicated in several cancers, making it a potential biomarker and therapeutic target. As a member of the APC/C, CDC27 interacts with various co-activators and substrates, influencing cell cycle progression and cellular responses to DNA damage. Recent studies focus on elucidating the mechanistic pathways involving CDC27, particularly in the context of cancer biology and treatment resistance. Understanding CDC27's functional dynamics not only sheds light on fundamental cell cycle regulation but also paves the way for the development of novel targeted therapies aimed at restoring proper cell cycle control in tumorigenesis. This makes CDC27 a pivotal subject in cancer research, with ongoing investigations aimed at delineating its role in cellular homeostasis and its potential as a therapeutic target in cancer treatment.
