Analytical Data
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Gene name
PNO1
- Application
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Biological Activity
Measured in a cell proliferation assay using HCT116 cells. The ED50 for this effect is typically 0.4094-0.5623 μg/mL. Measured in a cell proliferation assay using HCT116 cells. The ED50 for this effect is typically 0.4094 μg/mL, corresponding to a specific activity is 2442.599 units/mg.
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Alternative Names
RNA-binding protein PNO1; Partner of NOB1
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9NRX1
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Expression Region
M1-F252
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AA Sequence
MESEMETQSARAEEGFTQVTRKGGRRAKKRQAEQLSAAGEGGDAGRMDTEEARPAKRPVFPPLCGDGLLSGKEETRKIPVPANRYTPLKENWMKIFTPIVEHLGLQIRFNLKSRNVEIRTCKETKDVSALTKAADFVKAFILGFQVEDALALIRLDDLFLESFEITDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTRTRIVLADVKVHILGSFQNIKMARTAICNLILGNPPSKVYGNIRAVASRSADRF
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Protein Length
Full Length
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Molecular Weight
32 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PNO1, also known as Partner of NOB1, is a crucial protein involved in the regulation of ribosome biogenesis and maturation, playing a significant role in cellular protein synthesis. Its importance has been highlighted in various studies linking it to critical cellular processes, including gene expression and cell proliferation. Recent research has also indicated that PNO1 is involved in certain pathophysiological conditions, such as cancer, where aberrations in ribosome biogenesis can lead to uncontrolled cell growth. Given its pivotal role, the recombinant expression of PNO1 has become a focal point for researchers aiming to elucidate its functional mechanisms and interactions within the ribosomal assembly pathway. Producing PNO1 as a recombinant protein offers several advantages, including the ability to obtain large quantities for in-depth biochemical characterization, structural studies, and the development of potential therapeutic targets. Additionally, studying PNO1 in a controlled environment allows for investigations into its binding partners and modulation effects, which could unveil novel insights into how disruptions in its function may contribute to disease states. Overall, the research on recombinant PNO1 not only enhances our understanding of ribosomal dynamics but also opens avenues for therapeutic intervention in diseases associated with ribosomal dysfunction.
