Analytical Data
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Gene name
G3BP1
- Application
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Biological Activity
Measured in a cell proliferation assay using HCT116 cells. The ED50 for this effect is 5.264 ng/mL, corresponding to a specific activity is 1.9×10^5 units/mg. Measured in a cell proliferation assay using HCT116 cells. The ED50 for this effect is 5.264 ng/mL, corresponding to a specific activity is 1.9×10^5 units/mg.
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Alternative Names
G3BP-1; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q13283-1
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Expression Region
M1-Q466
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Protein Length
Full Length of Isoform-1
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Molecular Weight
65-69 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
G3BP1, or GTPase-activating protein 1, is a crucial protein that plays an essential role in various cellular processes, including stress granule formation, viral replication, and regulation of gene expression. It is particularly significant in the context of the cellular stress response, where it facilitates the assembly of stress granules—cytoplasmic ribonucleoprotein complexes that sequester mRNAs and prevent their translation during unfavorable conditions. Given its involvement in key regulatory mechanisms, G3BP1 has attracted attention in both basic and applied research fields, especially in studies related to neurodegenerative diseases, cancer, and viral infections. The role of G3BP1 in mediating responses to cellular stress underscores its potential as a therapeutic target. Recent advancements in recombinant protein technology have enabled researchers to produce G3BP1 in vitro, allowing for detailed structural and functional analyses. By studying the characteristics and interactions of recombinant G3BP1, scientists aim to unravel its specific roles in cellular homeostasis and pathophysiology. Understanding G3BP1's mechanisms may lead to novel therapeutic approaches for diseases where cellular stress responses are disrupted.
